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Standardization of a Cytometric Bead Assay Based on Egg-Yolk Antibodies
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Standardization of a Cytometric Bead Assay Based on Egg-Yolk Antibodies
DOI:

10:23 min

May 19, 2023

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Chapters

  • 00:04Introduction
  • 00:45Extraction of IgY from Egg Yolks
  • 02:27Saturation Curve of IgY Antibodies to Latex Beads
  • 04:02Flow Cytometry Assay Based on Latex Beads for Antigen Detection
  • 05:05Flow Cytometer Reading of the Samples and Sample Analysis
  • 07:33Results: Electrophoretic Profile of the IgY‐PfHRP2 Antibody
  • 09:29Conclusion

Summary

自动翻译

This protocol describes a methodology for the preparation of latex beads for assays using IgY antibodies for antigen detection.

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