Ultra-Fast Amplicon-Based Next-Generation Sequencing in Non-Squamous Non-Small Cell Lung Cancer

Christophe Bontoux; Virginie Lespinet-Fabre; Olivier Bordone; Virginie Tanga; Maryline Allegra; Myriam Salah; Salom&#233; Lalv&#233;e; Samantha Goffinet; Jonathan Benzaquen; Charles-Hugo Marquette; Marius Ili&#233;; V&#233;ronique Hofman; Paul Hofman

doi:10.3791/65190

JoVE Journal
Biology

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JoVE Journal Biology

Ultra-Fast Amplicon-Based Next-Generation Sequencing in Non-Squamous Non-Small Cell Lung Cancer

基于扩增子的超快速二代测序在非鳞状非小细胞肺癌中的应用

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07:59 min

September 8, 2023

DOI: 10.3791/65190-v

Christophe Bontoux^1,2,3,4,5, Virginie Lespinet-Fabre^1,2,3,4, Olivier Bordone^1,2,3,4, Virginie Tanga^1,2,3,4, Maryline Allegra^1,2,3,4, Myriam Salah^1,2,3,4, Salomé Lalvée^1,2,3,4, Samantha Goffinet^1,2,3,4, Jonathan Benzaquen^3,4,5,6, Charles-Hugo Marquette^3,4,5,6, Marius Ilié^1,2,3,4,5, Véronique Hofman^1,2,3,4,5, Paul Hofman^1,2,3,4,5

¹Laboratory of Clinical and Experimental Pathology, Centre Hospitalier Universitaire de Nice,Université Côte d’Azur, CHU de Nice, ²Hospital-Integrated Biobank (BB-0033-00025),Université Côte d'Azur, Hôpital Pasteur, CHU de Nice, ³Institut Hospitalo-Universitaire (IHU), RespirERA,Université Côte d'Azur, Hôpital Pasteur, CHU de Nice, ⁴FHU OncoAge,Université Côte d'Azur, ⁵Team 4, Institute of Research on Cancer and Aging (IRCAN), CNRS INSERM, Centre Antoine-Lacassagne,Université Côte d'Azur, ⁶Department of Pulmonary Medicine and Thoracic Oncology, Centre Hospitalier Universitaire de Nice,Université Côte d'Azur

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Overview

This study addresses the urgent need for rapid detection of genomic alterations in non-squamous non-small cell lung cancer (NS-NSCLC) to inform targeted therapies. An ultra-fast next-generation sequencing (NGS) workflow is presented as a solution for efficient analysis of small biopsy samples.

Key Study Components

Research Area

Oncology
Molecular diagnostics
Next-generation sequencing

Background

Targeted therapies for NS-NSCLC depend on timely detection of genomic alterations.
Traditional biopsy methods often yield small samples, complicating analysis.
A comprehensive approach for rapid testing is needed to enhance patient care.

Methods Used

Ultra-fast next-generation sequencing (NGS)
Analysis of formalin-fixed paraffin-embedded (FFPE) samples
Automated purification and sequencing processes

Main Results

The workflow identifies key genetic mutations and fusions in a time-efficient manner.
High sensitivity for detecting various genomic alterations was achieved.
Comprehensive molecular profiling was performed on 259 NS-NSCLC patients.

Conclusions

This study demonstrates a new standard for rapid genomic profiling in NS-NSCLC.
It provides critical insights for personalized treatment strategies in oncology.