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3D Culture of Organoids from Murine Intestinal Crypts and Single Stem Cell

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Author Spotlight: The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research

JoVE 杂志
生物学

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JoVE 杂志生物学

The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research

Author Spotlight: The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research

3D Culture of Organoids from Murine Intestinal Crypts and Single Stem Cell

DOI:

10.3791/65219-v

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02:35 min

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April 07, 2023

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Yuta Takase, Kazuto Fujishima³, Toshio Takahashi

¹Suntory Foundation for Life Sciences,Bioorganic Research Institute, ²Institute for Integrated Cell-Material Sciences (KUIAS-iCeMS),Kyoto University, ³Faculty of Medicine,Osaka Medical and Pharmaceutical University

Tags

3D Organoids Intestinal Crypts Intestinal Stem Cells Homeostasis Epithelial Growth Differentiation EDTA Mechanical Dissociation Signaling Intestinal Epithelium Paneth Cells Enteroendocrine Cells Enterocytes Goblet Cells Tuft Cells Leucine-rich Repeats Containing G Protein-coupled Receptor 5

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