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DOI: 10.3791/65234-v
Please note that some of the translations on this page are AI generated. Click here for the English version.
This study focuses on understanding the effects of linoleic acid on lipid droplet size and number in liver cells, aiming to provide insights into the mechanisms underlying fatty liver development. Key methodologies include the use of Oil Red O staining and live cell imaging to observe lipid droplet fusion.
本协议描述了如何使用油红O染色脂滴(LDs),计算脂肪酸诱导的脂肪肝细胞模型中LDs的大小和数量,并使用BODIPY 493 / 503观察小LDs通过活细胞成像融合成大LD的过程。
我们的研究重点是脂滴数和亚油酸诱导的脂质液滴数量和大小在肝细胞中。此外,我们的目标是提供对脂肪肝机制的见解。该研究领域的最新进展表明,胆碱,CCT α,建立诱导自噬,可以调节牛肝细胞中的脂滴大小。
此外,活细胞工作站的观察表明,磷脂对脂滴融合有相当大的影响。目前,基因辅助、荧光镊子、液相色谱、组学分析、单色谱、流式细胞术等技术正被用于对这一领域的大量研究进行分组。实验方法有一些局限性。
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