Low-Cost Electroencephalographic Recording System Combined with a Millimeter-Sized Coil to Transcranially Stimulate the Mouse Brain <em>In Vivo</em>

Takahiro Yoshikawa; Hiromu Sato; Koki Kawakatsu; Takashi Tateno

doi:10.3791/65302

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Low-Cost Electroencephalographic Recording System Combined with a Millimeter-Sized Coil to Transcranially Stimulate the Mouse Brain In Vivo

低成本脑电图记录系统结合毫米级线圈，在体内经颅刺激小鼠大脑

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05:26 min

May 26, 2023

DOI: 10.3791/65302-v

Takahiro Yoshikawa*¹, Hiromu Sato*¹, Koki Kawakatsu¹, Takashi Tateno²

¹Bioengineering and Bioinformatics, Graduate School of Information Science and Technology,Hokkaido University, ²Bioengineering and Bioinformatics, Division of Information Science and Technology,Hokkaido University

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Overview

This study proposes a low-cost electroencephalographic (EEG) recording system combined with a millimeter-sized coil for driving transcranial magnetic stimulation (TMS) in vivo in mice. The system aims to advance understanding of brain activity through multi-site recordings in response to TMS, enabling researchers to explore excitability and connectivity across brain regions.

Key Study Components

Area of Science

Neuroscience
Electrophysiology
Transcranial Magnetic Stimulation (TMS)

Background

TMS and EEG are used to study brain function non-invasively.
Simultaneous TMS-EEG measurements enhance exploration of brain dynamics.
Existing systems for small animals have limitations and require custom solutions.
This study develops a practical, adaptable system for standard labs.

Purpose of Study

To create a simultaneous stimulation measurement system for TMS-EEG in small animals.
To improve accessibility and practicality for neuroscience research.
To clarify mechanisms underlying neurological disorders using small animal models.

Methods Used

Electroencephalography (EEG) recording with a flexible multielectrode array.
Mouse model under intraperitoneal anesthesia for electrode and coil placement.
Detailed surgical and setup procedures for optimal placement of electrodes and stimulation device.
Recording and stimulation sessions using a function generator and data acquisition system.

Main Results

Successful recordings of EEG waveforms in response to sound and TMS.
Negative going responses observed post-sound stimulation, with variations based upon magnetic stimulation intensity.
Increased peak amplitudes in EEG recordings correlated with heightened stimulation.
Data illustrates the system's capability to measure brain responses during TMS.

Conclusions

The study demonstrates a practical setup for simultaneous TMS-EEG in rodents.
This methodology opens avenues for further research on cerebral mechanisms related to disorders.
Improved understanding of human neurological conditions and potential treatments is anticipated.

Frequently Asked Questions

What are the advantages of this TMS-EEG setup?

The low-cost and adaptable nature of the setup makes it available for standard neuroscience labs, facilitating simultaneous measurement of brain activity during TMS.

How is the mouse model prepared for the experiment?

Mice are intraperitoneally anesthetized, and the skull is exposed to attach electrodes and appropriately place the TMS coil.

What types of outcomes are measured using this method?

The system enables recordings of EEG waveforms, capturing brain responses to both sound and magnetic stimulation.

Can the setup be adapted for other types of studies?

Yes, the described system is versatile and can be modified to suit various experimental needs in neuroscience research.

What electrical parameters are measured during the experiments?

Conductance between electrodes and connectors is measured to ensure proper electrical function before recordings begin.

What limitations should researchers consider with this setup?

Challenges may arise from the need for customization to specific experimental conditions, as well as potential variability in animal responses.

提出了一种低成本的脑电图记录系统，结合毫米尺寸的线圈来驱动体内小鼠大脑的经颅磁刺激。使用具有定制、柔性、多电极阵列基板的传统螺旋电极，可以从小鼠大脑响应经颅磁刺激进行多位点记录。

在神经科学中，TMS和EEG记录经常被用来促进我们对大脑的理解。TMS由于其非侵入性而特别有前途。将TMS与EEG记录相结合，使研究人员能够探索不同大脑区域的兴奋性，连通性和时空动力学。