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DOI: 10.3791/65312-v
Please note that some of the translations on this page are AI generated. Click here for the English version.
该协议提出了一种优化的分离叶生物测定系统,用于评估昆虫病原真菌(EPF)对孤雌生殖昆虫芥菜蚜虫(Lipaphis erysimi (Kalt.))的有效性。该方法概述了培养皿实验期间的数据收集过程,使研究人员能够始终如一地测量EPF对芥菜蚜虫和其他孤雌生殖昆虫的毒力。
如今,环保害虫管理已广受青睐。然而,在实施之前,昆虫病原真菌必须经过彻底的测试。我们的研究展示了昆虫病原真菌库在对抗芥菜蚜虫方面的有效性,这是其潜力的一个典型例子。
降低昆虫病原真菌预处理的高死亡率和预防孢子悬浮液具有挑战性。我们已经成功地开发了一个稳定的系统,产生了预期的结果。这涉及使用流行的系统或生物测定室,以在测试过程中有效地维护这种微小的害虫。
该协议成功地展示了一种无需测量琼脂温度即可设置分离叶方法的可行方法,从而简化了实验室的建立。此外,我们提出了尽量减少琼脂表面暴露的重要性,以防止蚜虫在腔室内漫游时卡住。我们对芥菜蚜虫在蚜虫死亡之前面对昆虫病原真菌时的免疫反应很感兴趣。
通过研究基因表达数据,我们可以结合昆虫病原真菌应用,确定可以作为基因敲低潜在靶标的关键基因。
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