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JoVE Journal
Immunology and Infection
使用狂犬病间接荧光抗体检测狂犬病 IgG 和 IgM 抗体
使用狂犬病间接荧光抗体检测狂犬病 IgG 和 IgM 抗体
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Detection of Rabies IgG and IgM Antibodies Using the Rabies Indirect Fluorescent Antibody Test

使用狂犬病间接荧光抗体检测狂犬病 IgG 和 IgM 抗体

Full Text
3,364 Views
07:54 min
January 19, 2024

DOI: 10.3791/65459-v

Nicholas J. Jones1, Jodie A. Jarvis1, Kim A. Appler1, April D. Davis1

1Rabies Laboratory, Wadsworth Center,New York State Department of Health

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Please note that some of the translations on this page are AI generated. Click here for the English version.

本手稿的目的是研究使用狂犬病间接荧光抗体检测来检测狂犬病特异性 IgG 和 IgM 抗体。

IFA 测试评估狂犬病感染期间的抗体反应或评估疫苗的免疫反应。它可用于确定样品中是否存在抗体,并区分存在哪些抗体同种型。中和抗体检测是狂犬病抗体检测的金标准,用于评估接受过暴露前或暴露后预防的患者的抗体滴度。

尽管IFA检测可能产生与中和抗体检测不同的结果,但作为替代方法时,它仍然可以提供有价值的信息。通常,测量狂犬病中和抗体的检测方法会评估免疫反应。然而,这些测试需要数天时间,需要病毒和活细胞培养。

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