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DOI: 10.3791/65547-v
Please note that some of the translations on this page are AI generated. Click here for the English version.
本文提供了一种使用 Bradford 测定法和智能手机作为分析设备进行蛋白质定量的方案。样品中的蛋白质水平可以使用从智能手机拍摄的微孔板图片中提取的颜色数据进行量化。
我们的研究重点是在生化水平上了解动物如何适应极端环境条件。我们探讨了它们对缺氧和抑制代谢率的反应问题。具体来说,我们研究了动物在不利环境中使用的内源性抗氧化剂、氧化应激和细胞应激反应。
为此,我们经常量化样品中的蛋白质水平。该协议解决了在资源有限的情况下获得蛋白质定量数据的替代方法的需求,使其成为教育和研究环境中的宝贵工具。RG Bradford 检测试剂盒为蛋白质浓度测量提供了一种实用且可靠的解决方案,在无法使用酶标仪等传统设备的情况下,该解决方案易于访问、简单且准确。
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