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Biochemistry
RGBradford:使用智能手机相机进行蛋白质定量
RGBradford:使用智能手机相机进行蛋白质定量
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
RGBradford: Protein Quantitation with a Smartphone Camera

RGBradford:使用智能手机相机进行蛋白质定量

Full Text
5,019 Views
07:41 min
September 8, 2023

DOI: 10.3791/65547-v

Daniel C. Moreira1

1Research Center in Morphology and Applied Immunology, Faculty of Medicine,University of Brasilia

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Please note that some of the translations on this page are AI generated. Click here for the English version.

本文提供了一种使用 Bradford 测定法和智能手机作为分析设备进行蛋白质定量的方案。样品中的蛋白质水平可以使用从智能手机拍摄的微孔板图片中提取的颜色数据进行量化。

我们的研究重点是在生化水平上了解动物如何适应极端环境条件。我们探讨了它们对缺氧和抑制代谢率的反应问题。具体来说,我们研究了动物在不利环境中使用的内源性抗氧化剂、氧化应激和细胞应激反应。

为此,我们经常量化样品中的蛋白质水平。该协议解决了在资源有限的情况下获得蛋白质定量数据的替代方法的需求,使其成为教育和研究环境中的宝贵工具。RG Bradford 检测试剂盒为蛋白质浓度测量提供了一种实用且可靠的解决方案,在无法使用酶标仪等传统设备的情况下,该解决方案易于访问、简单且准确。

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生物化学 第199期 智能手机相机 分析仪器 考马斯亮蓝染料 颜色数据 微孔板 图像分析软件 蓝绿色强度 未知浓度的蛋白质 标准曲线 吸光度数据

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