Surgical Porcine Model of Chronic Myocardial Ischemia Treated by Exosome-laden Collagen Patch and Off-pump Coronary Artery Bypass Graft

Rishav Aggarwal; Annie Shao; Koray N. Potel; Laura Hocum Stone; Cory Swingen; Christin Wright; Edward O. McFalls; Tammy A. Butterick; Rosemary F. Kelly

doi:10.3791/65553

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JoVE Journal Medicine

Surgical Porcine Model of Chronic Myocardial Ischemia Treated by Exosome-laden Collagen Patch and Off-pump Coronary Artery Bypass Graft

外泌体胶原贴片和体外循环冠状动脉旁路移植术治疗慢性心肌缺血的外科猪模型

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05:25 min

September 15, 2023

DOI: 10.3791/65553-v

Rishav Aggarwal¹, Annie Shao¹, Koray N. Potel², Laura Hocum Stone¹, Cory Swingen¹, Christin Wright¹, Edward O. McFalls³, Tammy A. Butterick^4,5, Rosemary F. Kelly¹

¹Department of Surgery,University of Minnesota, ²School of Medicine, Dentistry and Biomedical Sciences,Queen’s University Belfast, ³Cardiology,Richmond VA Medical Center, ⁴Department of Research Service, Center for Veterans Research and Education,Veterans Affairs Health Care System, ⁵Department of Neuroscience,University of Minnesota

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本研究提出了进行性冠状动脉狭窄导致的慢性心肌缺血的外科猪模型，导致心脏功能受损而无梗死。缺血后，动物接受非体外循环冠状动脉旁路移植术，心外膜放置干细胞来源的富含外泌体的胶原蛋白贴片。这种辅助治疗可改善心肌功能和恢复。

该外科手术的总体目标是使用 LIMA 到 LAD 搭桥创建冠状动脉血运重建的手术模型，并使用富含外泌体的胶原蛋白贴片作为辅助手段来证明心脏功能的生理改善。我们的体内模型是单血管模型，不包括任何合并症，这不能代表接受多血管疾病治疗的患者的典型临床情况。然而，尽管存在这种局限性，我们的模型始终如一地产生了可靠的结果，并保持了较低的死亡率。

我们开发了一种临床相关的辅助疗法，用于 CABG，旨在了解驱动慢性心肌缺血心肌功能改善的细胞机制。缺血组织的完全恢复似乎取决于线粒体能力的恢复和炎症的减少;代表了一个新的研究领域。

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