Tracing <em>de novo</em> Lipids using Stable Isotope Labeling LC-TIMS-TOF MS/MS

Katherine D. Castro; Lilian Valadares Tose; Matthew Willetts; Melvin A. Park; Marcela Nouzova; Fernando G. Noriega; Francisco Fernandez-Lima

doi:10.3791/65590

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Chemistry

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Tracing de novo Lipids using Stable Isotope Labeling LC-TIMS-TOF MS/MS

使用稳定同位素标记 LC-TIMS-TOF MS/MS 从头追踪脂质

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07:12 min

August 23, 2024

DOI: 10.3791/65590-v

Katherine D. Castro¹, Lilian Valadares Tose¹, Matthew Willetts², Melvin A. Park², Marcela Nouzova³, Fernando G. Noriega^4,5, Francisco Fernandez-Lima^1,6

¹Deparment of Chemistry and Biochemistry,Florida International University, ²Bruker Daltonics Inc., ³Institute of Parasitology,Biology Centre CAS, ⁴Department of Biological Sciences,Florida International University, ⁵Department of Parasitology,University of South Bohemia, ⁶Biomolecular Sciences Institute,Florida International University

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这里介绍的是一种利用其保留时间、迁移率和碎裂通过稳定同位素标记来筛选脂质结构的方法。

本研究旨在使用稳定同位素标记在脂质组学样品中实现更特异性的定量。这使我们能够了解脂质面板和从昆虫到人类的高度多样化的样品。目前，该领域在脂质组学研究中 LC、TIMS、Top、MS/MS 的标准化方面存在差距。

在这里，我们提供了一种为此目的跟踪生物量遗骸的方法。未来，我们的研究将涉及使用 LC 团队工作人员 MS/MS 对生物过程中存在的表观遗传学和代谢组学进行调查，涉及 DDA 和 DIA 方法。

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