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Biology
用于真菌叶面玉米病原体感染的活细胞成像的分离玉米鞘
用于真菌叶面玉米病原体感染的活细胞成像的分离玉米鞘
JoVE Journal
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JoVE Journal Biology
Detached Maize Sheaths for Live-Cell Imaging of Infection by Fungal Foliar Maize Pathogens

用于真菌叶面玉米病原体感染的活细胞成像的分离玉米鞘

Full Text
2,059 Views
06:12 min
September 15, 2023

DOI: 10.3791/65755-v

Renata Belisário1, Maria F. Torres1,2, Ester A. S. Buiate1,3, Katia V. Xavier1,4, Etta M. Nuckles1, Lisa J. Vaillancourt1

1Department of Plant Pathology,University of Kentucky, 2Department of Biological Sciences,University of Cincinnati, 3Bayer Crop Science, 4Everglades Research and Education Center,University of Florida

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Summary

这份手稿详细介绍了一种优化的接种方案,该方案使用分离的玉米叶鞘对玉米与真菌植物病原体的相互作用进行可重复的细胞学、生理学和分子学研究。叶鞘有助于实时观察未固定组织中活植物和真菌之间的细胞相互作用。

Transcript

因此,我们的团队旨在了解植物中的真菌致病机制,以改善疾病管理。我们目前正在探索玉米本土真菌如何调节蛋白质分泌,而不仅仅是其管家功能。我们的方案显示出复制之间的高度同步性和真菌定植的可重复性。

此外,使用我们的检测方法,无需在实时成像之前清除或固定样品。该方法在植物-病原体相互作用中提供了多种应用,并有助于对玉米真菌致病机制的详细研究和比较基因组分析。

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分离的玉米鞘 活细胞成像 真菌叶面玉米病原体 方案优化 半生物营养真菌 坏死真菌 显微镜观察 真菌生长发育 活植物细胞 孢子悬浮液 湿度室 连续荧光灯 表皮细胞 光学清晰度 实时可视化 染色 浆液溶解 发育细胞学 宿主和病原体细胞的活力 荧光蛋白 竞争性相互作用 协同相互作用 荧光融合蛋白

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