Tracking miRNA Release into Extracellular Vesicles using Flow Cytometry

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07:29 分钟

2023年10月6日

10.3791/65759-v

2023年10月6日

2.1K 次观看

Here, we describe a straightforward protocol that enables in vitro assessment of the abundance of fluorescently labeled microRNAs to study the dynamics of microRNA packaging and export into extracellular vesicles (EVs).

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RNA RNA RNA Calu 6

Chapters in this video

0:00

Introduction

1:32

Flow Cytometry Analysis of Cell‐miRNA and EV‐miRNA Signal in Calu6 Cells Transfected with Fluorophore‐Conjugated EV‐miRNA

4:28

Flow Cytometry Analysis of Cell‐miRNA and EV‐miRNA Signal in EVs Isolated from Calu6 Cells Transfected with Fluorophore‐Conjugated EV‐miRNA

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