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Medicine
肺部感染性疾病的微生物学快速现场评估
肺部感染性疾病的微生物学快速现场评估
JoVE Journal
Medicine
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JoVE Journal Medicine
Microbiological Rapid On-Site Evaluation for Pulmonary Infectious Diseases

肺部感染性疾病的微生物学快速现场评估

Full Text
665 Views
03:22 min
March 1, 2024

DOI: 10.3791/66059-v

Xiangwen Weng*1, Weiting Sun*2, Zhenchun Luo*1, Yuling Zhou1, Xing An3

1Department of Emergency and the Intensive Care Unit,Chongqing Hospital of Traditional Chinese Medicine, 2Department of Critical Care Medicine,Hospital of Chengdu University of Traditional Chinese Medicine, 3Respiratory and critical care,Chongqing Hospital of Traditional Chinese Medicine

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Please note that some of the translations on this page are AI generated. Click here for the English version.

Summary

这里的方案展示了一种快速和标准化的微生物快速现场评估 (M-ROSE) 工作流程,包括三个步骤:载玻片制作、染色和解释。该协议将帮助医生快速做出临床决策。

Transcript

我们的目的是引入一种实时快速评估方法 M-ROSE,以帮助医生为肺部感染患者制定准确的治疗策略。快速细胞染色技术和高清显微成像系统目前用于推进该领域的研究。关于 M-ROSE 的临床研究数量相对有限。

最大的挑战是 M-ROSE 结果的解释,这需要人员和训练有素的人员,因为很难目视识别病原体并区分感染和污染。通过更好地了解实时引起感染的病原体,医生可以避免使用不必要的广谱抗生素,这些抗生素会导致抗生素耐药性并用于治疗肺部感染。M-ROSE 是实时且高效的。

M-ROSE 能够对病原体进行显微镜鉴定,例如曲霉菌、隐球菌、许多和念珠菌。它在确定呼吸基本质量、区分感染与非感染性疾病、区分感染与污染以及评估感染、严重程度和预后方面具有重要的指导意义。

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关键词:快速现场评估 肺部传染病 抗感染治疗 痰培养 支气管肺泡灌洗 基因测序 经验性治疗 抗生素耐药性 M-ROSE工作流程 靶向抗感染治疗 个体化药物治疗

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