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Bioengineering
将静态屏障组织模型转化为动态微生理系统
将静态屏障组织模型转化为动态微生理系统
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Transforming Static Barrier Tissue Models into Dynamic Microphysiological Systems

将静态屏障组织模型转化为动态微生理系统

Full Text
1,527 Views
06:20 min
February 16, 2024

DOI: 10.3791/66090-v

Mehran Mansouri1, Aidan R. Hughes1, Lauren A. Audi1, Anna E. Carter1, Justin A. Vidas1, James L. McGrath2, Vinay V. Abhyankar1

1Department of Biomedical Engineering,Rochester Institute of Technology, 2Department of Biomedical Engineering,University of Rochester

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Please note that some of the translations on this page are AI generated. Click here for the English version.

该协议描述了一种可重新配置的基于膜的细胞培养平台,该平台将开孔形式与流体流动功能集成在一起。该平台与标准方案兼容,并允许在开孔和微流控培养模式之间进行可逆过渡,以满足工程和生物科学实验室的需求。

我们正在开发模拟人体屏障组织的模块化微生理系统。我们的系统是独一无二的,因为它可以从静态明井培养重新配置为流动增强系统。这种灵活性使该平台可用于生物科学和工程实验室。

我们提出了一种协议,用于创建具有流动增强文化和生活方式成像的可重新配置的明井平台。它与传统的生物科学协议兼容。我们的可重构设计允许用户在实验期间在开孔和微流控模式之间切换,并允许用户使用他们熟悉的格式进行每个步骤。

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