Identifying Bone Marrow Microenvironmental Populations in Myelodysplastic Syndrome and Acute Myeloid Leukemia

Christina M. Kaszuba; Benjamin J. Rodems; Sonali Sharma; Edgardo I. Franco; John M. Ashton; Laura M. Calvi; Jeevisha Bajaj

doi:10.3791/66093

JoVE Journal
Cancer Research

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JoVE Journal Cancer Research

Identifying Bone Marrow Microenvironmental Populations in Myelodysplastic Syndrome and Acute Myeloid Leukemia

识别骨髓增生异常综合征和急性髓系白血病中的骨髓微环境群体

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06:33 min

November 10, 2023

DOI: 10.3791/66093-v

Christina M. Kaszuba^1,2, Benjamin J. Rodems^1,3, Sonali Sharma^1,3, Edgardo I. Franco^1,2, John M. Ashton^1,3,4, Laura M. Calvi^1,5, Jeevisha Bajaj^1,3

¹Wilmot Cancer Institute,University of Rochester Medical Center, ²Department of Biomedical Engineering,University of Rochester, ³Department of Biomedical Genetics,University of Rochester Medical Center, ⁴Genomics Research Center,University of Rochester Medical Center, ⁵Division of Endocrinology and Metabolism, Department of Medicine,University of Rochester Medical Center

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本文介绍了从骨髓增生异常综合征和急性髓系白血病的小鼠模型中分离和表征骨髓微环境群体的详细方案。该技术可识别非造血骨髓生态位（包括内皮细胞和间充质基质细胞）随疾病进展的变化。

我们的研究旨在探索骨髓不断变化的环境如何影响肿瘤的生长。我们开发了一种流式细胞术方案来分析骨髓和骨组织中的非免疫细胞，重点关注小鼠血液系统恶性肿瘤。该方法适用于研究各种小鼠模型中的骨髓。

我们的研究重点是分离和识别癌症进展过程中骨髓基质群的改变。了解它们对疾病发生和进展的影响可以为针对骨髓基质细胞中这些促进癌症变化的新疗法铺平道路。我们的方案独特地结合了机械和酶消化、磁耗竭和流式细胞术来描述骨髓微环境细胞组成。

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