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JoVE Journal
Immunology and Infection
一种在野外和实验室条件下诊断人畜共患线虫的简单粪便浮选方法
一种在野外和实验室条件下诊断人畜共患线虫的简单粪便浮选方法
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A Simple Fecal Flotation Method for Diagnosing Zoonotic Nematodes Under Field and Laboratory Conditions

一种在野外和实验室条件下诊断人畜共患线虫的简单粪便浮选方法

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4,092 Views
03:46 min
December 15, 2023

DOI: 10.3791/66110-v

Julio Segura1, Yazmin Alcala-Canto1, Antonio Figueroa1, Victor Del Rio1, Guillermo Salgado-Maldonado2

1Parasitology Department, Facultad de Medicina Veterinaria y Zootecnia,Universidad Nacional Autonoma de Mexico, 2Instituto de Biología,Universidad Nacional Autonoma de Mexico

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Please note that some of the translations on this page are AI generated. Click here for the English version.

这项工作描述了使用浮选方法鉴定 2017 年至 2021 年在野外条件下从墨西哥狗身上收集的粪便样本中检测到 的犬弓蛔虫 和 Ancylostoma spp.。

目前,正在对狗的人畜共患寄生虫进行全国范围的地理定位研究,以进一步控制和预防这些疾病。墨西哥存在各种各样的生态系统,这影响了狗寄生虫感染的频率。因此,有必要进行实地工作并识别这些寄生虫,以便能够根据每种寄生虫的流行病学提出针对特定地区的驱虫指南。

简单的浮选技术不够灵敏,无法检测隐孢子虫卵母细胞、贾第鞭毛虫囊肿或滴虫病。我们提出了一种浓缩程序,以更好地对这些寄生虫进行共寄生虫检测。这种技术可以加快和促进在没有实验室基础设施或设备的地区对狗的蠕虫感染的诊断。

每次显微镜检查后,可以立即开具检查结果和驱虫建议。该协议有助于在现场或实验室条件下进行常规粪便检查,以估计狗犬弓蛔虫和犬弓形虫感染的频率。

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