使用远端中动脉闭塞技术诱导小鼠急性缺血性卒中

Overview

This study presents a detailed protocol for establishing a distal middle cerebral artery occlusion (dMCAO) model in C57BL/6J mice using transcranial electrocoagulation. It focuses on evaluating the resulting neurological behaviors and histopathological features observed post-occlusion.

Key Study Components

Area of Science

• Neurological behavior assessment
• Histopathological analysis
• Ischemic stroke modeling

Background

• The medial cerebral artery (MCA) is key in ischemic strokes in humans.
• Various MCA occlusion methods exist, but dMCAO offers advantages in terms of surgical invasiveness and survival rates.
• The model is beneficial for exploring long-term functional recovery.

Purpose of Study

• To generate a reliable experimental ischemic stroke model in rodents.
• To assess behavioral outcomes and histopathological changes post-stroke.
• To utilize the model for future studies on cognition impairment.

Methods Used

• Transcranial electrocoagulation was employed to induce dMCAO.
• C57BL/6J mice were used to study neurological changes following occlusion.
• Behavioral tests such as grip strength, pole test, adhesive removal, and cylinder test were utilized.
• Histological examination of brain tissue was conducted post-behavioral assessments.

Main Results

• Mice exhibited significant reductions in grip strength and increased descent times on various behavioral tests.
• Histological analysis showed disordered neuron arrangements and reduced cell density in the peri-infarct area.

Conclusions

• The study establishes a robust dMCAO model with high reproducibility for stroke research.
• This methodology will enhance understanding of structural and behavioral outcomes after ischemic stroke.
• Future applications could focus on cognitive impairments in post-stroke recovery.

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