A Set of <em>In Situ</em> Informed Simulated Medium Formats for Culturing Environmentally Acquired Anaerobic Microorganisms

Tia Zimmerman; Garion Leamon; Georgia Dillenburg; Bethany Egge; Jennifer Pierce; Ben Elliott; Trevor Murphy; Marjorie Brooks; Scott D. Hamilton-Brehm

doi:10.3791/66228

JoVE Journal
Environment

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JoVE Journal Environment

A Set of In Situ Informed Simulated Medium Formats for Culturing Environmentally Acquired Anaerobic Microorganisms

一套用于培养环境获得性厌氧微生物的原位信息模拟培养基格式

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07:56 min

January 12, 2024

DOI: 10.3791/66228-v

Tia Zimmerman¹, Garion Leamon¹, Georgia Dillenburg¹, Bethany Egge¹, Jennifer Pierce², Ben Elliott³, Trevor Murphy¹, Marjorie Brooks⁴, Scott D. Hamilton-Brehm¹

¹Department of Microbiology,Southern Illinois University Carbondale, ²MEDPREP,Southern Illinois University School of Medicine, ³Department of Geology,Southern Illinois University Carbondale, ⁴Department of Zoology,Southern Illinois University Carbondale

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本文的重点是详细说明为从环境中获得的挑剔厌氧微生物制作培养基的最佳实践。这些方法有助于管理厌氧培养物，并可用于支持难以捉摸的未培养微生物（"微生物暗物质"）的生长。

我们的研究重点是陆地表面和地下微生物，培养它们并研究它们的基因组和表型。特别关注的是尚不清楚的环境中的微生物，例如深层地下和某些湿地中的微生物。我们培养的大多数微生物都是厌氧的，这意味着培养过程通常比更常用的需氧微生物需要更多的时间和精力。

我们希望厌氧培养基制备的视听演示能够帮助刚接触该技术的研究人员相对快速和容易地学习它。这种培养基制备基于 Miller 和 Wolin 的改良 Hungate 技术。培养挑剔的微生物既乏味又有问题。

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