Measuring Bacterial Colonization on <i>Arabidopsis thaliana</i> Roots in Hydroponic Condition

Xia Shu; Huatai Li; Jing Wang; Shan Wang; Yunpeng Liu; Ruifu Zhang

doi:10.3791/66241

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Measuring Bacterial Colonization on Arabidopsis thaliana Roots in Hydroponic Condition

在水培条件下测量拟南芥根上的细菌定植

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05:37 min

March 1, 2024

DOI: 10.3791/66241-v

Xia Shu^1,2, Huatai Li¹, Jing Wang³, Shan Wang⁴, Yunpeng Liu¹, Ruifu Zhang^1,3

¹State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China, Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences, ²State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology,Huazhong Agricultural University, ³Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization,Nanjing Agricultural University, ⁴Jiangsu Engineering and Technology Center for Modern Horticulture,Jiangsu Vocational College of Agriculture and Forestry

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植物促生长根际（PGPR）在根际的定植对其促生长作用至关重要。有必要规范细菌根际定植的检测方法。在这里，我们描述了一种可重复的方法，用于量化细菌在根表面的定植。

我们的研究重点是有益根际细菌与植物根系的相互作用。我们正在寻找增强有益菌定植的关键机制，从而提高田间微生物肥料的效率和稳定性。细菌在植物根系定植的方法已经得到了广泛的研究，但仍然存在问题。

例如，很难对不同位点的定植进行不同的测量，我们认为在未来应用显微镜和高通量计数将有助于解决问题。我们的方案具有高度适用性和良好的可重复性，更适合于准确的根际细菌定植测定。

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