单色多重定量聚合酶链式反应端粒长度测量
3.4K 次观看
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被引用 7 次
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11:44 分钟
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2024年3月22日
DOI :10.3791/66545-v
2024年3月22日
•3.4K 次观看
Noelle A. Martin*1 , Lauren W. Y. McLester-Davis*2,3,4 , Trevor R. Roy1 , Madeline G. Magruder5 , Waylon J. Hastings1 , Stacy S. Drury6
1School of Medicine, Tulane University, 2Native American Center for Health Professions, University of Wisconsin-Madison, 3Department of Medicine, University of Wisconsin-Madison, 4Department of Biochemistry, University of Wisconsin-Madison, 5School of Science and Engineering, Tulane University, 6Department of Psychiatry and Behavioral Sciences, Boston Children’s Hospital
* These authors contributed equally
本文介绍了一种利用单色多重定量聚合酶链式反应（MMqPCR）检测法测定相对端粒长度（TL）的实验方案。MMqPCR检测法是一种可重复、高效且经济的方法，适用于在基于人群的研究中测定人源DNA的端粒长度。
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Chapters in this video
0:00
Introduction
1:49
Sample and Master Mix Preparation for Telomere Length Measurement Using Monochrome Multiplex Quantitative PCR (MMqPCR)
3:42
Preparation of 96‐Well Plate for Monochrome Multiplex Quantitative PCR (MMqPCR) for Telomere Length Measurement
6:21
Monochrome Multiplex Quantitative PCR (MMqPCR) Data Analysis to Check the Reproducibility of Telomere Length Measurements
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