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02:30 min
January 10, 2025
DOI:
10.3791/66619-v
我们的研究探讨了骨组织环境干扰的影响。我们对了解模拟微重力和振动对成人和骨骼发育的影响特别感兴趣。我们使用斑马鱼作为首选的模式生物,因为它为生物研究提供了许多优势。
与以前的方案相比,我们的方案具有优势,因为它限制了对二氧化碳培养箱的需求,并且它使用标准实验室设备。我们的实验室正试图解开不同类型骨细胞在受到环境干扰(如重力或振动)时的通信动力学。未来,我们希望能够减轻太空旅行对骨组织的负面影响。
该方案提供了一种使用简单的 体外 培养技术研究细胞动力学的方法。它为斑马鱼研究人员和教育工作者提供了一个机会,通过可视化鳞片内的荧光细胞核和凋亡细胞,研究细胞过程,例如与骨稳态和基本细胞生物学相关的细胞过程。
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Cite this Article
Carvajal-Agudelo, J. D., Franz-Odendaal, T. A. In Vitro Culturing Technique for Studying Cellular Dynamics in Zebrafish Scales. J. Vis. Exp. (215), e66619, doi:10.3791/66619 (2025).
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