用于确定狨猴卵巢周期阶段的血液采样和激素测量
July 5th, 2024
该方案描述了用于测量孕酮/雌二醇和绒毛膜促性腺激素水平以确定卵巢周期阶段的血液和尿液采样。激素水平用于预测和确定排卵的时间，并注射激素以调节卵巢周期和卵母细胞生长。
我们正在两个主要方面开展工作。首先，我们的目标是了解灵长类动物（包括人类）的雄性生殖细胞。我们专注于精原干细胞和 DNA 甲基化的建立。
其次，我们的目标是开发制造转基因狨猴的新技术。转基因狨猴的产生需要付出很多努力。挑战之一是开发更有效的方法来制造转基因狨猴。
我们的血液采样方案可以更轻松地找到静脉并降低从动脉抽血的风险。此外，我们的方案还测量了三种类型的激素，并带来了更准确的排卵日期预测和确定。我们现在正在开发一种从狨猴的 iPS 细胞制造精子的技术。
这项技术将有助于生殖细胞的研究和转基因狨猴的产生。
概述
本研究调查灵长类动物（包括人类）的雄性生殖细胞，重点关注精原细胞干细胞及其DNA甲基化过程。此外，研究旨在开发创建转基因卷尾猴的改进方法，包括一个增强静脉通路并便于测量激素水平以预测排卵的血液采样方案。
主要研究组成部分
研究领域
- 雄性生殖细胞生物学
- 基因修改技术
- 卵巢周期调节
背景
- 了解雄性生殖细胞对生殖生物学至关重要。
- 转基因卷尾猴可以提供对人类疾病的见解。
- 准确的排卵时间有助于生殖管理。
使用的方法
- 采集血液和尿液测量孕酮、雌二醇和绒毛膜促性腺激素水平。
- 卷尾猴作为生物模型生物。
- 用于观察激素水平和开发转基因生物的创新方法。
主要结果
- 改进的血液采样方法提高了激素测量的精确度。
- 基于激素水平的排卵时间预测得到改善。
- 在卷尾猴iPS细胞中产生精子细胞方面取得进展。
结论
- 该研究展示了对雄性生殖细胞的理解和增强基因修改技术的进展。
- 这些发现对灵长类生物的生殖生物学和基因研究具有重要意义。
常见问题
Executive Industry Relevance
Accurate staging of the ovarian cycle in marmosets using quantitative hormone measurement is critical for enabling precise timing in reproductive and genetic modification workflows. This capability directly impacts the reliability of oocyte and zygote collection, supporting translational research and the development of genetically modified primate models. The protocol enhances predictive confidence at key inflection points in reproductive and developmental biology pipelines.
Strategic Applications in Biopharma R&D
Early Discovery & Target Validation
- Enables functional validation of reproductive targets through precise hormone quantification.
- Supports mechanistic de-risking by clarifying ovarian cycle stage and hormonal milieu.
- Facilitates portfolio triage by improving timing and success rates in genetic modification studies.
Screening & Assay Development
- Standardizes blood and urine sampling for reproducible hormone assays in non-human primates.
- Delivers quantitative outputs for progesterone, estradiol, and chorionic gonadotropin levels.
- Prepares validated biological systems for downstream reproductive and developmental workflows.
Translational & Preclinical Research
- Aligns reproductive staging with translational biomarker strategies in primate models.
- Enables continuity from discovery through preclinical validation in reproductive and genetic studies.
- Reduces biological risk in the generation of genetically modified marmosets for disease modeling.
Pipeline & Workflow Integration
This protocol integrates into the discovery-to-preclinical continuum by providing a standardized approach for hormone measurement and ovarian cycle staging in marmosets.
- Discovery Biology: Supports hypothesis testing and biological de-risking in reproductive research.
- Screening: Ensures assay readiness and reproducibility for hormone quantification.
- Analytics: Provides quantitative hormone readouts to compare physiological states and optimize timing.
- Translational Research: Bridges discovery and preclinical work by enabling reliable model generation.
- Enterprise Reuse: Establishes a reusable protocol for hormone measurement across reproductive and developmental studies.
Operational & Enterprise Impact
- Scientific Value: Increases predictive confidence and reduces mechanistic ambiguity in reproductive workflows.
- Operational Value: Enhances standardization, reproducibility, and scalability of hormone assays in primate models.
- Strategic Value: Improves go/no-go decisions and capital efficiency in genetic modification programs.
- Portfolio Impact: Enables risk-adjusted prioritization and advancement of reproductive and developmental projects.
Implementation Considerations
- Requires expertise in primate handling and blood sampling techniques.
- Needs access to validated hormone assay platforms and analytical infrastructure.
- Demands cross-team standardization for sample collection and data interpretation.
- May require adaptation for different primate species or reproductive contexts.
- Dependent on precise timing and physiological monitoring as supported by the protocol.
