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Biochemistry
生物测定 - 通过薄层色谱法 - 直接生物自闭法鉴定用于生物防治的天然产物
生物测定 - 通过薄层色谱法 - 直接生物自闭法鉴定用于生物防治的天然产物
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Bioassay-Guided Identification of Natural Products for Biocontrol by Thin Layer Chromatography-Direct Bioautography

生物测定 - 通过薄层色谱法 - 直接生物自闭法鉴定用于生物防治的天然产物

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2,245 Views
04:52 min
July 26, 2024

DOI: 10.3791/66967-v

Leah Gauthier1,2, Brian D. Wagner2, Sue Boyetchko3, Christopher W. Kirby1,2

1Charlottetown Research and Development Centre,Agriculture and Agri-Food Canada, 2Department of Chemistry,University of Prince Edward Island, 3Saskatoon Research and Development Centre,Agriculture and Agri-Food Canada

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Please note that some of the translations on this page are AI generated. Click here for the English version.

我们描述了使用薄层色谱、直接生物自闭测定法和液相色谱-质谱法来识别使用病原体菌核 病 菌和生物 农药芽孢 杆菌分离株作为模型系统来识别对真菌病原体表现出拮抗作用的微生物天然产物。

在农业中,关于发现合成杀虫剂替代品的研究在过去二十年中取得了显著增长。通过检查生物活性土壤微生物产生的天然产物,我们的团队设想发现环境中已经存在的新生物杀虫剂。层析直接生物统计法或 TLCDB 主要用于确定植物提取物对病原菌和真菌的生物活性。

在本视频中,无需诱导病原体变质即可对细菌提取物进行检测,从而可以检查更广泛的病原体。TLCBD 将继续成为我们用来专注于发现新型天然产物的工具之一 对于生物控制,这将使农业和农业食品部门受益,并有助于减轻病原体对商业杀虫剂的耐药性。

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