Establishing a Severe Corneal Inflammation Model in Rats Based on Corneal Epithelium Curettage Combined with Corneal Sutures

Xiaoyu Tian; Meng Zhang; Yiming Wu; Liying Zhang; Lingli Zhang; Xueer Zheng; Shangkun Ou; Hao Gu

doi:10.3791/67305

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JoVE Journal Medicine

Establishing a Severe Corneal Inflammation Model in Rats Based on Corneal Epithelium Curettage Combined with Corneal Sutures

基于角膜上皮刮除术联合角膜缝合术的大鼠重度角膜炎症模型

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04:48 min

November 22, 2024

DOI: 10.3791/67305-v

Xiaoyu Tian*^1,2,3, Meng Zhang*^1,2, Yiming Wu⁴, Liying Zhang^1,2, Lingli Zhang^1,2, Xueer Zheng^1,2, Shangkun Ou^1,2, Hao Gu^1,2

¹Department of Ophthalmology,the Affiliated Hospital of Guizhou Medical University, ²School of Clinical Medicine,Guizhou Medical University, ³The Second People's Hospital of Guiyang City, ⁴Department of Biomedical Sciences, School of Infection, Inflammation and Immunology, College of Medicine and Health,University of Birmingham

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我们通过角膜上皮刮除术联合角膜缝合开发了严重角膜炎症大鼠模型。该研究评估了炎症条件下角膜皮炎症模式、上皮增殖和角膜缘干细胞的变化。

欢迎建立数星细胞缺陷的可更新模型，我们通过角膜上皮刮片联合角膜缝合成功建立了由严重信息引起的数星细胞缺陷模型。由于红色角膜的厚度只有人眼死亡的三分之一，缝合角膜表面很容易引起穿孔，这对我们的手术技术提出了很高的要求。该模型在不直接损伤边缘上皮干细胞的情况下诱导角膜炎症，从而促进角膜炎症对边缘上皮干细胞的影响和机制的研究。

这有助于观察严重炎症对 limbo 干细胞生物学机制的影响。首先，在工作平台上布置手术器械，包括消毒的角膜除锈环剂、皮肤取样标记、持针器、板层刀、眼科手术剪刀和镊子，选择 7 至 8 周龄的雌性 Sprague 多利大鼠，并在裂隙灯下验证是否存在眼表病变。麻醉后，大鼠使用 iota four 对眼睛周围的皮肤和毛发进行消毒。

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