Pregnancy and Nursing Management for Embryo-Transferred and Genetically Modified Rabbits

Bangzhu Chen; Chula Sa; Guiling Li; Tianping Liu; Ke Liu; Weiwang Gu; Gang Wang

doi:10.3791/67793

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Biology
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JoVE Journal Biology

Pregnancy and Nursing Management for Embryo-Transferred and Genetically Modified Rabbits

胚胎转移和转基因兔的妊娠和哺乳管理

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04:29 min

December 13, 2024

DOI: 10.3791/67793-v

Bangzhu Chen*^1,2, Chula Sa*³, Guiling Li², Tianping Liu³, Ke Liu², Weiwang Gu³, Gang Wang²

¹Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, The Third Affiliated Hospital,Guangzhou Medical University, ²Guangdong Medical Laboratory Animal Center, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences),Southern Medical University, ³Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering,Wuyi University

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Overview

This study details a pregnancy and nursing management technique specifically for embryo-transferred and genetically modified rabbits, aiming to significantly reduce newborn mortality and enhance the preparation efficiency of gene-edited rabbits. By implementing refined management practices, this protocol addresses common challenges faced by beginners in rabbit breeding.

Key Study Components

Research Area

Genetic modification
Animal husbandry
Rabbit breeding techniques

Background

High mortality rates in newborn rabbits
Challenges in preparing gene-edited rabbits
Need for improved breeding management strategies

Methods Used

Embryo transfer procedures
Palpation techniques for pregnancy detection
Use of hormone injections (cloprostenol, oxytocin)

Main Results

Improved survival rate of newborn rabbits
Refined management practices enhance breeding success
Effectiveness of induced labor and nursing techniques

Conclusions

This study demonstrates the effectiveness of a structured breeding protocol.
Findings are relevant for advancing genetic research in rabbits.

Frequently Asked Questions

What is the goal of the pregnancy management technique?

To reduce the mortality rate of newborn rabbits and improve the efficiency of gene-edited rabbit preparation.

What are the main methods involved in this technique?

Techniques include embryo transfer, pregnancy palpation, and hormone injections for labor induction.

How does this technique differ from traditional methods?

It involves more refined management and is tailored for genetically modified rabbits, requiring additional time and effort.

What role does nutrition play in this management technique?

Adequate nutrition for the mother rabbit is crucial for the health and survival of the offspring.

At what time frame should hormone injections be administered?

Hormones like cloprostenol are injected based on fetal counts around the 30th day of pregnancy to induce labor.

How are newborn rabbits cared for immediately after birth?

Newborns are cleaned and placed in an incubator to ensure proper breathing and warmth.

What signs indicate that the mother rabbit is ready to deliver?

Signs include restlessness, rapid breathing, and nesting behavior.

该方案描述了一种胚胎移植和转基因兔的妊娠和喂养管理技术，旨在降低新生兔死亡率并提高基因编辑兔的制备效率。

我们描述了一种胚胎转移兔和转基因兔的妊娠和哺乳管理技术，旨在降低幼兔的死亡率并增强基因编辑兔的制备效果。初学者通常很难成功制备基因编辑的兔子，他们经常遇到怀孕率低、流产、难产等令人不快的问题。兔妈妈拒绝母乳喂养，经常有小兔子死亡。

与兔场使用的传统自然分娩和养育方法相比，这种方法涉及更精细的管理，需要额外的时间和精力，但显着提高了新生兔的存活率。首先，获得一只通过移植程序接收胚胎的兔子。用一只手将兔子的脖子皮肤放在耳朵后面，将它的臀部向前朝向腹腔。

用另一只手轻轻触诊腹部后端以检测怀孕迹象。胚胎移植后的第 25 天，将培养箱固定在分娩笼的一侧，并在里面放置毛巾、刨花或纸屑，为怀孕的兔子创造一个巢。在笼子里提供足够的饲料和饮用水，轻轻地将怀孕的兔子移动到准备好的分娩笼中。

然后，在怀孕第 30 天，用一只手将兔子的颈部皮肤放在耳后，将兔子的臀部向前朝向腹腔。用另一只手轻轻触诊腹部后端，以评估胎儿的状况。如果怀孕的兔子只怀上了 1 到 2 个胎儿，请在下午 5：00 肌肉注射 0.02 毫克氯前列醇，以诱导第二天分娩。

如果兔子表现出分娩迹象，例如烦躁不安、呼吸急促、耳血管扩张或筑巢行为，请肌肉注射 10 单位的催产素以引产。接下来，安排并准备培养箱。向培养箱中添加无菌吸收性填充物，例如玉米芯颗粒。

铺上毛巾作为垫子，并用人造羽绒棉覆盖。用毛巾快速擦去新生兔子身上的粘液，确保呼吸顺畅，将洗净的兔子放入培养箱中。清理完后，记下后代的数量。

产后，使用浸有酒精的棉花，在第一次喂食前清洁母兔的所有。轻轻按摩每个奶嘴，挤出第一滴奶。将兔妈妈以侧卧或俯卧姿势放在垫子上，并逐一引导 1 至 2 日龄的兔宝宝到吸吮乳汁。

出生后的第二天，用记号笔在每只小兔子的背部做记号。要喂养两天以上的小兔子，请将母兔放入保温箱中独立母乳喂养。幼兔在大约两周大时睁开眼睛后，将它们移到衬有毛巾的哺乳笼中。

将母兔与幼兔分开后，每天打开挡板一次进行母乳喂养。让母兔在哺乳期间自由饮水和进食，并为它提供适当的绿色饲料以确保足够的营养。

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