Minimally Invasive Cisterna Magna Injection Model for Leptomeningeal Metastasis Studies in Mice

Xiaoyi Wang; Tie Tong; Qingsheng Xu; Yuxiang Weng; Weijie Zhang

doi:10.3791/68190

JoVE Journal
Cancer Research

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JoVE Journal Cancer Research

Minimally Invasive Cisterna Magna Injection Model for Leptomeningeal Metastasis Studies in Mice

用于小鼠软脑膜转移研究的微创 Cisterna Magna 注射模型

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07:14 min

May 23, 2025

DOI: 10.3791/68190-v

Xiaoyi Wang¹, Tie Tong², Qingsheng Xu¹, Yuxiang Weng¹, Weijie Zhang³

¹Department of Neurosurgery, the First Affiliated Hospital, School of Medicine,Zhejiang University, ²Life Sciences Institute,Zhejiang University, ³Zhejiang Key Laboratory of Molecular Cancer Biology, Life Sciences Institute, and Department of Orthopaedic Surgery, the Second Affiliated Hospital, School of Medicine,Zhejiang University

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该方案描述了一种通过经皮穿刺途径将肿瘤细胞注射到大脑池的方法，该方法可强烈诱导小鼠软脑膜转移，减少创伤和颅外肿瘤负荷。

软脑膜转移，是一种非常严重和致命的转移性疾病。其环境可能取决于肿瘤细胞与独特的组织微环境之间的相互作用。动物模型，研究潜在机制非常重要。

为了在体内研究软脑膜转移，研究人员通常使用心内、颈动脉内或将癌细胞直接注射到大池或脑室等技术。这些方法有助于为 LM 研究生成可靠的小鼠模型。目前的尝试有明显的局限性。例如，心内注射通常会在软脑膜间隙外产生肿瘤，并可能导致与 LM 无关的死亡。而颈动脉入路是高度侵入性和耗时的。该协议可以快速生成 LM 模型。它绕过血脑屏障，将肿瘤细胞精确地输送到软脑膜间隙，减少手术创伤和宝贵时间。

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