利用液滴界面双层膜表征脂质-蛋白质膜的结构与功能
461 次观看
•
10:27 分钟
•
2026年6月12日
DOI :10.3791/70628-v
2026年6月12日
•461 次观看
Peter T. Podar1,2 , Ariana Adkisson-Washington2,3 , Olivia Ziemer2,4 , Victoria Sullivan2,5 , Christian Milem2,6 , Janine Hagar-Montoya2,7 , Shriya Khule2,8 , Zachary Griffith2,9 , Khalid Tantawi2,10 , Xiuhong Zhai2,11 , Dima Bolmatov12 , John Katsaras2,11 , Charles Patrick Collier13
1Carle Illinois College of Medicine, University of Illinois at Urbana-Champaign, 2Shull Wollan Center, University of Tennessee, 3Department of Chemistry, Louisiana State University, 4Department of Physics and Astronomy, University of Tennessee, 5Department of Chemical and Biomolecular Engineering, University of Tennessee, 6Department of Chemical and Environmental Engineering, University of Cincinnati, 7Department of Metallurgical, Materials, and Biomedical Engineering, University of Texas at El Paso, 8Farragut High School, 9Department of Chemical and Biological Engineering, University of Alabama, 10Department of Engineering Management and Technology, University of Tennessee, 11Labs and Soft Matter Group, Oak Ridge National Laboratory, 12Department of Physics and Astronomy, Texas Tech University, 13Center for Nanophase Materials Sciences, Oak Ridge National Laboratory
本文介绍了一种实验方案，用于组装、电刺激并分析掺有短杆菌肽A的液滴界面双层膜。通过测量膜面积、离子流和单通道电导的变化，并将这些响应与受电突触启发的膜模型中类似可塑性的离子传导变化相关联，从而量化脂质-蛋白质的结构-功能关系。
Chapters in this video
0:00
Introduction
0:28
Formation of Droplet Interface Bilayers (DIBs)
2:50
Electrical Setup and Bilayer Monitoring
5:13
Electrical Stimulation Protocol for Paired-Pulse Facilitation and Depression
8:02
Results
9:41
Conclusion
Related Videos
版权所有 © 2026 MyJoVE Corporation。保留所有权利。