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Biology
在人类胚胎干细胞体外培养的标签用于磁共振成像
在人类胚胎干细胞体外培养的标签用于磁共振成像
JoVE Journal
Biology
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JoVE Journal Biology
In vitro Labeling of Human Embryonic Stem Cells for Magnetic Resonance Imaging

在人类胚胎干细胞体外培养的标签用于磁共振成像

Full Text
10,608 Views
11:41 min
August 3, 2008

DOI: 10.3791/827-v

Mayumi Yamada1, Phillip Yang1

1Division of Cardiovascular Medicine,Stanford University

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Please note that some of the translations on this page are AI generated. Click here for the English version.

在这段视频中,我们展示了如何标注人类胚胎干细胞(胚胎干细胞)与氯化锰(MnCl

磁共振成像 (MRI) 已成为局部移植干细胞的主要成像方式,可以更好地了解再生机制。这些 MRI 研究通常使用氧化铁纳米颗粒作为成像造影剂。然而,铁颗粒在干细胞死亡后会留在组织中,并可能被巨噬细胞吸收,从而导致追踪活移植细胞的条件不理想。

本视频将证明氯化锰是一种有效的替代品。与氧化铁标记相比,用氯化锰进行造影剂标记具有多项优势。例如,锰发出正信号,而负信号由离子粒子产生。

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