Name: punnett squares for predicting traits
Uploaded: 2019-03-11T09:21:16.000Z
Duration: 1 min
Channel: JoVE

K12 Standards

Virginia  Standards of Learning

HIgh School

Life Science

LS.10

LS.10.c

Punnett Squares

Das Punnett Quadrat, ein einfaches Diagramm, das nach seinem Erfinder benannt wurde, ist eine grafische Methode, um die Wahrscheinlichkeit vorherzusagen, dass Nachkommen aufgrund der elterlichen Genotypen ein einzelnes Genmerkmal wie eine Laktoseintoleranz erben.
 Unter Verwendung dieses Gitters werden die genetischen Beiträge eines Elternteils, der in diesem Fall homozygot rezessiv mit Allelen ist, bezeichnet mit zwei kleingeschriebenen L, oben plaziert.
 Die des anderen, heterozygot mit gemischten Allelen, dargestellt durch ein großes L zusammen mit einem rezessiven kleinem l, werden auf die linke Seite geschrieben.
 Die Kombination dieser Zeilen- und Spaltenüberschriften in den Kästchen führt zu zwei möglichen Genotypen und beiden Phänotypen für ein Kind.
 Eine 50% ige Chance, heterozygot zu sein und Laktose verdauen zu können, und eine 50% ige Chance, homozygot rezessiv und laktoseintolerant zu sein.

Edwards, A. W. F. "Reginald Crundall Punnett: Erster Arthur-Balfour-Professor für Genetik, Cambridge, 1912." Genetics 192, Nr. 1 (1. September 2012): 3. [<a href="https://doi.org/10.1534/genetics.112.143552" target="__blank">Quelle</a>]

Reid, James B. und John J. Ross. "Mendels Gene: Auf dem Weg zu einer vollständigen molekularen Charakterisierung." Genetics 189, Nr. 1 (1. September 2011): 3. [<a href="https://doi.org/10.1534/genetics.111.132118" target="__blank">Quelle</a>]

punnett-quadrate

<h4>Überblick</h4>
 
 Ein Punnett-Quadrat (Rekombinationsquadrat) zeigt die möglichen Genotypen, die Nachkommen von zwei elterlichen Genotypen erben können. Wenn das Vererbungsmuster eines Merkmals (z.B. dominant oder rezessiv) bekannt ist, können Punnett-Quadrate auch zur Bestimmung der Wahrscheinlichkeit der Vererbung eines Phänotyps verwendet werden. Punnett-Quadrate sind in Situationen einsetzbar, in denen die Vererbung von Merkmalen durch einen einzelnen Genlocus bestimmt wird und Merkmale unabhängig voneinander vererbt werden. Sie können jedoch nicht die Wahrscheinlichkeit von Merkmalen für komplexere genetische Vererbungsszenarien voraussagen.
 
 <h4>Punnett-Quadrate zeigen die Wahrscheinlichkeit der Vererbung einer oder mehrerer Eigenschaften an</h4>
 
 Punnett-Quadrate sind visuelle Darstellungen der möglichen Genotypen der Nachkommen, die aus einer Kreuzung zweier elterlicher Genotypen resultieren. Sie können die Vererbung von einem oder mehreren Phänotypen oder Merkmalen darstellen. Andere Werkzeuge sind jedoch besser geeignet, wenn es darum geht, die Vererbung von mehr als zwei Merkmalen zu untersuchen.
 
 Punnett-Quadrate können verwendet werden, um die Wahrscheinlichkeit zu bestimmen, dass Nachkommen einen bestimmten Genotyp oder ein Paar von Allelen erben, die ein bestimmtes Merkmal (d.h. Phänotyp oder Merkmal) verursachen. Hierbei wird vorausgesetzt, dass der Phänotyp durch einen einzelnen Genlocus verursacht wird und während der Meiose unabhängig voneinander weitergegeben wird. Punnett-Quadrate sind also nützlich zur Bestimmung von Vererbungswahrscheinlichkeiten in Szenarien, in denen die Wahrscheinlichkeit, ein Merkmal zu erben, die Wahrscheinlichkeit, ein anderes zu erben, nicht beeinflusst. Obwohl es viele Ausnahmen zu diesen Annahmen gibt (z.B. Merkmale, die von benachbarten Genen auf demselben Chromosom kodiert werden), gelten diese Qualifizierungsmerkmale für viele pflanzliche und tierische Merkmale.
 
 <h4>Mendels Experimente inspirierten die Erstellung der Punnett-Quadrate</h4>
 
 Punnett-Quadrate wurden in den frühen 1900er Jahren von Reginald Punnett, einige Jahrzehnte nach Gregor Mendels erstellt. Die bahnbrechenden Erbsenpflanzenexperimente enthüllten die grundlegenden Gesetze der Vererbung. Heute werden Punnett-Quadrate oft zur Veranschaulichung der Prinzipien verwendet, denen Mendels Experimente zugrunde liegen.
 
 Mendel untersuchte die Vererbung verschiedener Merkmale der Erbsenpflanze. Dazu gehörten Form und Farbe der Erbsen und Schoten, der Blütenfarbe und-position sowie Pflanzengröße. Mendel untersuchte auch die vererbten Möglichkeiten für jedes Merkmal. Blüten können beispielsweise die Farben violett und weiß annehmen. Bei Erbsenpflanzen werden die violetten und weißen Blüten durch verschiedene Genvarianten oder Allele im Genlocus der Blütenfarbe bestimmt.
 
 <h4>Punnett-Quadrate sind Raster, die genetische Informationen organisieren</h4>
 
 Jede Box in einem Punnett-Quadrat repräsentiert ein mögliches Befruchtungsereignis oder einen möglichen Genotyp der Nachkommen, der sich aus zwei elterlichen Gameten ergeben kann. Punnett-Quadrate sind typischerweise in 2x2-oder 4x4-Konfigurationen angeordnet. So kann man die Vererbung von einem bzw. zwei Merkmalen visualisieren.
 
 Was die Nomenklatur betrifft, werden Allele durch den ersten Buchstaben des Merkmals, das durch das dominante Allel verursacht wird, gekennzeichnet. Da z.B. Gelb das dominante Farbmerkmal der Erbse ist, werden Allele, die die Erbsenfarbe kodieren, mit dem kursiv gedruckten Buchstaben lsquo;g. rsquo gekennzeichnet. Groß-und Kleinbuchstaben stehen für dominante bzw. rezessive Allele. So repräsentiert Y das dominante gelbe Allel und y das rezessive grüne Allel.
 
 Um zur Untersuchung eines Merkmals ein 2x2 Punnett-Quadrat zu erstellen, wird ein elterlicher Genotyp über dem Diagramm aufgelistet. Dabei fasst jede Spalte ein Allel zusammen. Der andere elterliche Genotyp wird vertikal links im Diagramm angezeigt, wobei ein Allel neben jeder Zeile steht. Jedes Punnett-Quadrat enthält die beiden elterlichen Allele (eines von jedem Elternteil), die der Zeile und Spalte des Diagramms entsprechen, und ein mögliches Befruchtungsergebnis darstellt. Der vollständige Inhalt der Punnett-Quadrate kann verwendet werden, um die Wahrscheinlichkeit der Vererbung bestimmter Merkmale zu bestimmen.
 
 <h4>Punnett-Quadrate sind informative Werkzeuge für genetische Ratgeber und Züchter</h4>
 
 Trotz der Entwicklung vor über 100 Jahren haben die Punnett-Quadrate immer noch einige relevante Anwendungsbereiche. Für ein Paar, das eine genetische Beratung erhält, können Punnett-Quadrate helfen, zu bestimmen, ob ihr Kind ein erhöhtes Risiko für eine vererbbare Krankheit hat. Wenn zum Beispiel ein Elternteil Mukoviszidose (zwei rezessive, kausale Allele) hat und der andere Elternteil sie weder hat noch trägt, wird ihr Kind ein Träger (d.h., es hat ein kausales Allel) sein, aber hat kein Risiko Mukoviszidose zu entwickeln. Punnett-Quadrate können Tier- und Pflanzenzüchtern auch helfen, Organismen mit spezifischen Merkmalen für die weitere Zucht auszuwählen.
 
 Obwohl Punnett-Quadrate in vielen Zusammenhängen nützlich sind, können sie die komplexe genetische Vererbung nicht genau abbilden. Zum Beispiel werden Merkmale, die von benachbarten Genen auf demselben Chromosom kodiert werden, oft gemeinsam von einem Elternteil vererbt, ein Phänomen, das als Kopplung bezeichnet wird. Diese Merkmale werden nicht unabhängig voneinander weitergegeben, so dass ein Punnett-Quadrat ihre Vererbungsmuster nicht genau vorhersagen kann. Einige Merkmale, wie z.B. die Körpergröße, sind für Punnett-Quadrate ungeeignet, da sie von mehreren Genen bestimmt und von Umweltbedingungen (z.B. der Ernährung) beeinflusst werden. Punnett-Quadrate sind auch unwirksam bei der Vorhersage der Vererbung von Merkmalen, die von nur einem Elternteil erworben wurden.

Punnett-Quadrate

Überblick

Ein Punnett-Quadrat zeigt die möglichen Genotypen, die Nachkommen von zwei elterlichen Genotypen erben können. Wenn das Vererbungsmuster eines Merkmals ...

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punnett squares for predicting traits

Punnett squares help predict which traits offspring may inherit from two parents. They show possible genotypes, which are pairs of alleles, and can also show the chance of a phenotype, or visible trait, when the inheritance pattern is known. These grids work best for traits controlled by a single gene locus and passed on independently during meiosis.

Punnett squares were developed in the early 1900s by Reginald Punnett. His work came after Gregor Mendel’s pea plant experiments revealed the basic laws of inheritance. Mendel studied traits such as pea and pod shape and color, flower color and position, and plant size. For flower color, purple and white are different traits controlled by different alleles at the same gene locus.

A Punnett square organizes genetic information in a grid. Each box represents one possible fertilization event, or one possible offspring genotype, from two parental gametes. These diagrams are often shown as 2x2 squares for one trait or 4x4 squares for two traits. Alleles are usually written with the first letter of the dominant trait. For pea color, Y stands for the dominant yellow allele, and y stands for the recessive green allele.

To build a 2x2 Punnett square, one parent’s genotype is placed across the top and the other parent’s genotype is placed down the side. One allele goes above each column, and one allele goes next to each row. The boxes then combine one allele from each parent. The full square shows the chances of each genotype and helps predict the trait an offspring may show.

Punnett squares still matter in genetics, counseling, and breeding. Genetic counselors can use them to estimate a child’s risk for inherited disease. For example, if one parent has cystic fibrosis and the other neither has nor carries it, the child will be a carrier but will not be at risk for cystic fibrosis. Breeders also use Punnett squares to choose organisms with useful traits for future breeding.

These grids do have limits. They do not work well for complex inheritance patterns, such as linked genes on the same chromosome, because those genes are not independently assorted. They are also not a good fit for traits like height, which can depend on several genes and environmental factors such as diet. Punnett squares are also not useful for traits acquired from only one parent.

Punnett Squares for Predicting Traits

genetic lingo

Genotype, Phenotype, and Inheritance

Genotype, phenotype, and inheritance help explain how traits and disease risk are passed from parents to offspring. In diploid organisms, including humans, most other animals, and many plants, somatic cells carry a duplicate set of chromosomes. These chromosome pairs are homologous, with similar length, centromere position, and gene locations. Diploid organisms inherit two versions of each gene, one from each parent. These versions are called alleles, and they sit at the same relative locus,...

punnett squares

Punnett squares help predict which traits offspring may inherit from two parents. They show possible genotypes, which are pairs of alleles, and can also show the chance of a phenotype, or visible trait, when the inheritance pattern is known. These grids work best for traits controlled by a single gene locus and passed on independently during meiosis. Punnett squares were developed in the early 1900s by Reginald Punnett. His work came after Gregor Mendel’s pea plant experiments revealed the...

monohybrid crosses

Mendel’s Pea Plant Inheritance Ratios

Gregor Mendel used pea plants to study how traits are passed from parents to offspring. His breeding experiments in the 1850s and 1860s showed that inheritance follows clear patterns. He crossed true-breeding plants that differed in one trait and found that dominant traits can hide recessive traits. Mendel focused on seven pea plant traits, and each trait had two forms controlled by a single gene locus. He noticed that true-breeding plants always produced offspring with the same phenotype, or...

dihybrid crosses

Mendel's Law of Independent Assortment

Mendel’s law of independent assortment explains how two traits can be inherited separately. Gregor Mendel tested this idea with pea plants that differed in two characteristics, such as pea color and pea shape. He used true-breeding parents that were homozygous for both traits but showed different phenotypes. In one example, Mendel crossed round, yellow peas with wrinkled, green peas. The parental generation had RRYY and rryy genotypes. Their first-generation offspring were all dihybrids,...

law of segregation

Mendel’s Pea Cross Ratios

Mendel’s pea plant crosses show how traits can disappear in one generation and return in the next. When he crossed plants, one parental trait sometimes seemed to vanish in the F1 generation and reappear in the F2 generation. Mendel used this pattern to explain why some traits are dominant and others are recessive. He proposed that traits are controlled by alleles, which are different versions of a gene. An organism inherits two copies of each gene, one from each parent. In his model, a...

law of independent assortment

Mendel’s Dihybrid Cross and Trait Ratios

Mendel’s dihybrid cross shows how two traits are inherited at the same time. A dihybrid cross is a cross in which both parents are homozygous for two distinct traits, and the F1 generation is heterozygous for both traits. Mendel used pea plants with round yellow peas and wrinkled green peas as an example. One parent had genotype YYRR, and the other had genotype yyrr. In the F1 generation, every plant showed the dominant traits, so the plants were YyRr and had yellow, round peas. The F2...

probability laws

Inheritance Risk with Sum and Product Rules

Inheritance risk can be calculated with the sum rule and the product rule. The sum rule applies to mutually exclusive events, or events that cannot happen at the same time. The product rule applies to independent events, or events that do not affect one another. These rules describe theoretical probability, which is the chance of an event before it happens. Empirical probability is different because it is based on events that have already occurred. These probability rules are useful in...

test cross

Using Test Crosses to Identify Genotypes

Test crosses are used to identify the genotype of an organism with a dominant trait. A genotype is the pair of alleles inherited for a gene, and alleles are different forms of the same gene. In diploid organisms, including humans, each gene usually has two alleles, one from each parent. An allele is recessive when its effect is hidden by another allele at the same gene location. Pea plants provide a clear example with flower color. White flowers are recessive, because one copy of the purple...

polygenic traits

Polygenic Traits in Human Height

Polygenic traits are traits controlled by more than one gene. Human height is a clear example of this kind of inheritance. Because many genes work together, the final trait can vary a lot from person to person. Researchers have found hundreds of loci, or gene locations, that influence height. There are likely many more that have not yet been identified. Many of these genes affect cartilage in the growth plates of the long bones in the arms and legs, either directly or indirectly. Height is...

pedigree analysis

Reading Family Traits in Pedigrees

Pedigrees are family trees used to track how a trait passes through generations. They help scientists and students see whether a trait is dominant or recessive, whether it is autosomal or sex-linked, what genotypes family members may have, and what phenotypes may appear in future generations. For families with a history of inherited disease, this information can be important for family planning. In plants and animals, inheritance is often studied with crosses. Monohybrid crosses can show...

multiple allele traits

Blood Type and Allele Interactions

Multiple alleles can shape traits through blood type, sickle cell anemia, incomplete dominance, and codominance. Multiple allelism means a gene has three or more allelic forms. Diploid organisms, including humans, usually carry only two alleles for each gene, but many human genes exist in multiple forms in the population. Human blood type is a clear example of multiple allelism. The blood type gene has three alleles: I A , I B , and i. These alleles help determine which blood type a person has...

epistasis

Gene Masking in Rabbit Fur Color

Epistasis describes how one gene can mask or change the effect of another gene. In high school genetics, this helps explain why many traits do not follow a simple single-gene pattern. Instead, alleles at different locations can work together to shape the final phenotype. Mendel studied seven unrelated pea traits, but most traits involve multiple gene interactions. Epistasis often happens when one gene interferes with the expression of another gene. It can also occur when genes are part of the...

nature and nurture

Genes and Environment Shape Height

Genes and environment both shape human height and other traits. Genes are the inherited instructions we get from our parents. Environment includes factors such as diet, stress, and social conditions. Many phenotypes, or observable traits, reflect both influences. Some also show up later in life, including cancer and other diseases. Height is a clear example of this interaction. Scientists estimate that 70-90% of height variation comes from genetic differences among people. The remaining 10-30%...

pleiotropy

SOX10 and Multiple Traits in Pleiotropy

Pleiotropy is when one gene affects more than one trait. The SOX10 gene is a clear example because it can influence pigmentation, hearing, and intestinal movement. These traits may seem unrelated, but they can all be linked to the same gene change. In humans, a deleted region in SOX10 can cause Waardenburg Syndrome Type 4, or WS4. WS4 is an autosomal recessive disorder, which means a person usually inherits two altered copies of the gene. Common signs include a white forelock,...

sex-linked disorders

X- and Y-Linked Disorder Patterns

X- and Y-linked disorders come from mutations in genes on the sex chromosomes. These genes, like those on autosomes, are needed for normal body function. When a mutation causes a biological problem, the condition is called sex-linked. Y-linked disorders come from changes in the Y chromosome. They affect only males, because only males carry a Y chromosome. Mutations in this small chromosome can affect male sexual function and secondary sex characteristics. One example is Y-chromosome...

x-linked traits

X-Linked Inheritance in Families

X-linked inheritance explains how traits on the X chromosome are passed through families. In most mammalian species, females have two X sex chromosomes, while males have one X and one Y. A mutation on one X chromosome may be masked in females by a normal allele on the other X. In males, that same mutation is more likely to show because there is no second normal X to balance it. Traits caused by changes on the X chromosome are called X-linked traits. Color blindness is a well-studied example.

x-inactivation

Barr Bodies and X-Chromosome Balance

Barr bodies and X-chromosome balance help explain how female mammalian cells control gene activity from the X chromosome. The human X chromosome carries far more genes than the Y chromosome. Because males usually have one X chromosome and females have two, females would make twice as many of some proteins without a control system. To prevent that imbalance, female mammalian cells inactivate nearly all the genes on one X chromosome during early embryonic development. The inactive X condenses...

non-nuclear inheritance

Maternal DNA in Mitochondria and Chloroplasts

Maternal DNA in mitochondria and chloroplasts explains how some genes are inherited outside the nucleus. Most DNA is in the nucleus, but mitochondria and chloroplasts in the cell cytoplasm also carry their own DNA and can copy it independently of nuclear DNA. Mitochondria are found in both plant and animal cells. They are called the powerhouses of eukaryotic cells because they break down glucose to make energy for cellular activity. Mitochondrial DNA has about 37 genes, and many help with...

chromosomal theory of inheritance

How Genes Map to Chromosomes

Genes map to chromosomes, and this idea explains how Mendel’s inheritance patterns work. Gregor Mendel published his pea plant breeding results in 1866. He showed that physical traits follow predictable patterns. At the time, scientists did not yet know that genes existed. Mendel used the word "factors" for the hereditary units he studied. The details behind Mendel’s laws of segregation and independent assortment were still unknown for many years. In the late 1800s, better microscopes and...

key terms and concepts

Learn how Punnett squares predict offspring genotypes and traits, from Mendel’s pea plants to genetic counseling, breeding, and simple inheritance patterns.

Punnett Squares for Predicting Traits | JoVE K12

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Next Generation Science Standards

High School

heredity-inheritance-and-variation-of-traits

Heredity: Inheritance and Variation of Traits

HS-LS3-1

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New York State Science Learning Standards

Inheritance and Variation of Traits

HS-LS3-1.

Georgia Standards of Excellence

9-12

SB3.b

Florida Department of Education

SC.912.L.16

SC.912.L.16.1

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Overview

An organism is diploid if it inherits two variants, or alleles, of each gene, one from each parent. These two alleles constitute the genotype ...

Overview

A Punnett square displays the possible genotypes offspring can inherit from two parental genotypes. If a trait&#x2019;s inheritance pattern ...

Monohybrid Crosses

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In the 1850s and 1860s, Gregor Mendel investigated inheritance by performing monohybrid crosses in pea plants. He crossed two plants that were ...

Dihybrid Crosses

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The student will investigate and understand that organisms reproduce and transmit genetic information to new generations. Key ideas include Punnett squares are mathematical models used to predict the probability of traits in offspring.

Punnett Squares for Predicting Traits

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