Name: ligand binding and allosteric effects
Uploaded: 2023-04-30T18:28:41.000Z
Duration: 49 s
Channel: JoVE

K12 Standards

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North Carolina K-12 Science Standard Course of Study

9-12

Biology

from-molecules-to-organisms-structures-and-processes

From Molecules to Organisms- Structures and Processes

LS.Bio.1.2

Ligand Binding and Linkage

Die meisten Proteine haben mehrere Stellen, an denen sich Liganden assoziieren können, zusätzlich zu der Stelle, die für die Proteinfunktion verantwortlich ist. Die Bindung eines Moleküls an eine dieser Stellen führt häufig zu Konformationsänderungen, die die Form des Proteins verändern.
Wenn diese Änderung die Bindung eines anderen Liganden beeinflusst, werden die beiden Stellen als gekoppelt oder verknüpft beschrieben. Die Bindungsstellenkopplung kann positiv oder negativ reguliert werden.
Bei einer positiven Kopplung führt die Bindung eines Liganden zu Konformationsänderungen, die eine Bindung an den jeweiligen Liganden an eine andere Stelle wahrscheinlicher machen. 
Bei einer negativen Kopplung führt die Bindung eines Liganden zu Konformationsänderungen, die verhindern, dass eine andere Bindungsstelle mit ihrem Liganden assoziiert wird.

<ol>
	<li>Suplatov, D., & Švedas, V. (2015). Untersuchung funktioneller und allosterischer Stellen in Protein-Superfamilien. Acta naturae, 7(4), 34–45.</li>
	<li>Bellelli, A. und Carey, J. (2017). Proteine mit mehreren Bindinstellen. In Reversible Ligandenbindung (Hrsg. A. Bellelli und J. Carey). doi:10.1002/9781119238508.ch4</li>
	<li>Helmstaedt, K., Krappmann, S., & Braus, G. H. (2001). Allosterische Regulation der katalytischen Aktivität: Escherichia coli Aspartat-Transcarbamoylase versus Hefe-Chorismat-Mutase. Mikrobiologie und Molekularbiologie Überprüfungen: MMBR, 65(3), 404–421. doi:10.1128/MMBR.65.3.404-421.2001</li>
</ol>

ligandenbindung und -kopplung

Allosterische Proteine besitzen mehr als eine Ligandenbindungsstelle; die Bindung eines Liganden an einer dieser Stellen beeinflusst die Bindung von Liganden an die anderen Stellen. Wenn ein Protein allosterisch ist, werden seine Bindungsstellen als gekoppelt oder verknüpft bezeichnet. Im Fall von Enzymen wird die Stelle, die das Substrat bindet, als aktive Stelle bezeichnet und die andere Stelle als regulatorische Stelle. Wenn ein Ligand an die regulatorische Stelle bindet, führt dies zu Konformationsänderungen im Protein, die die aktive Stelle des Proteins beeinflussen können.

Die Verknüpfung von Bindungsstellen kann entweder eine positive oder negative Regulation der Ligandenbindung an andere Stellen bewirken. In Fällen, in denen beide Liganden die gleiche Konformation eines Proteins bevorzugen, erhöht die Bindung an einer Stelle die Affinität der anderen Stelle für ihren jeweiligen Liganden. Dies wird als positive Verknüpfung bezeichnet. Andererseits, wenn die Liganden verschiedene Konformationen bevorzugen, erschwert die Bindung eines Liganden die Bindung eines zweiten Liganden an das Protein. Dies wird als negative Verknüpfung bezeichnet.

Ligandenbindung und -kopplung

Allosterische Proteine haben mehr als eine Ligandenbindungsstelle; Die Bindung eines Liganden an eine dieser Stellen beeinflusst die Bindung von Liganden an die andere ...

Education

Lehre

JoVE Core

Cell Biology

Zellbiologie

Unit 1

Einheit 1

Energy and Catalysis

Energie und Katalyse

KEY TERMS AND CONCEPTS

KERNBEGRIFFE UND KONZEPTE

ligand binding and allosteric effects

Ligand binding and linkage describe how one molecule can affect another when it binds to a protein. In this topic, the focus is on how binding at one site can influence activity at a different site on the same protein.

This idea is important for understanding protein function in high school biology. It helps explain why some proteins respond more strongly after a ligand attaches. The transcript centers on the connection between ligand binding and linkage, which is a key concept in molecular interactions.

The term ligand means a molecule that binds to a protein. Linkage describes the way that this binding is connected to changes elsewhere in the protein. Together, these ideas show how protein behavior can be controlled by molecular binding events.

Ligand Binding and Allosteric Effects

the first law of thermodynamics

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Learn how ligand binding can change protein activity through linkage, where binding at one site affects another site on the same protein.

Ligand Binding and Allosteric Effects | JoVE K12

cell biology

JoVE Core Zellbiologie veranschaulicht komplexe zelluläre Prozesse in leicht verständlichen und dennoch umfassenden animierten Videolektionen. Diese Sammlung konzentriert sich auf die wesentlichen Bestandteile und Funktionen von Zellen, einschließlich Organellen und Membranen, Zelltransport und Zellenergie. Ergänzt wird sie durch Videos von Wissenschaftlern in Aktion, die die Anwendung der Zellbiologie in realen Forschungsexperimenten demonstrieren.

Virginia  Standards of Learning

HIgh School

BIO.2

BIO.2.c

Florida Department of Education

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SC.912.L.18

SC.912.L.18.4

SC.912.L.18.11

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Ligand Binding and Allosteric Effects

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