Question 1

Why does temperature rise in a bioreactor during fermentation?

Accepted Answer

Temperature increases due to three primary sources: microbial metabolism generates heat as microorganisms break down nutrients, mechanical agitation from the impeller creates friction, and aeration introduces energy into the system. These exothermic processes combined can significantly elevate fermentation broth temperature, requiring active cooling to maintain optimal microbial growth conditions.

Question 2

What methods are used to control temperature in bioreactors?

Accepted Answer

Temperature control employs water baths, external jackets, or internal cooling coils that circulate refrigerated water to remove excess heat. Advanced systems use PID control with thermostatic water recirculation for precision within ±0.1°C. In laboratory settings, resistance thermometers and thermistors provide real-time monitoring, while larger systems rely on automated regulation to maintain optimal fermentation conditions.

Question 3

How does microbial metabolism affect pH in fermentation broth?

Accepted Answer

Microbial metabolic processes produce organic acids and other byproducts that alter fermentation broth pH, which directly impacts microbial growth and product yield. Ammonia is often used to neutralize acids produced during fermentation. Monitoring and controlling pH is essential because even small fluctuations can inhibit microbial activity and reduce fermentation efficiency.

Question 4

What control strategies maintain optimal pH in bioreactors?

Accepted Answer

pH is regulated using buffers or automated systems equipped with sterilizable probes that detect pH changes and trigger acid or base addition. Combined sterilizable electrodes, typically silver/silver chloride-based, provide continuous monitoring. Control systems range from simple on/off mechanisms to proportional-derivative controllers, enabling precise automated dosing to maintain target pH levels throughout fermentation.

Question 5

What causes foam formation in bioreactors and how is it managed?

Accepted Answer

Foam results primarily from aeration, agitation, or microbial activity. It is managed either mechanically using foam breakers or chemically using antifoams. Foam sensing typically involves probes that activate antifoam addition upon foam contact. Effective foam control prevents contamination, nutrient loss, and reduced product yield.

Question 6

What properties make an ideal antifoam for bioreactor use?

Accepted Answer

The ideal antifoam is non-toxic, autoclavable, non-metabolizable, and does not interfere with oxygen transfer. Antifoams reduce surface tension and break down foam films by disrupting bubble stability. These properties ensure that antifoams effectively control foam without compromising microbial growth, product quality, or oxygen availability in the fermentation system.

Question 7

How are dissolved oxygen and carbon dioxide monitored in bioreactors?

Accepted Answer

Oxygen availability is monitored using galvanic or polarographic electrodes, or newer optical sensors based on fluorescence quenching. Carbon dioxide levels are measured using infrared sensors, phenol red-based colorimetric methods, or thermal conductivity detectors. These measurements inform control systems that adjust air flow, impeller speed, or supplement pure oxygen to maintain required dissolved oxygen levels.

Bioreactor Controls-I

Browse More Videos