Gleichzeitige Ganzzellableitungen von Photorezeptoren und Neuronen zweiter Ordnung in eine Amphibie Retinal Scheibe Vorbereitung
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Summary
Abstract
Protocol
Representative Results
Discussion
Disclosures
The authors have nothing to disclose.
Acknowledgements
Materials
| Tissue slicer | Stoelting | 51425 | |
| Double edge razor blades | Ted Pella, Inc | 121-6 | |
| Nitrocellulose membranes | Millipore | AAWP02500 | Type AAWP 0.8 mm pore |
| Borosilicate glass pipettes | World Precision Instruments | TW120F-4 | 1.2mm OD 0.95 mm ID |
| Ag/AgCl pellet | Warner | E206 | |
| MicroFil | World Precision Instruments | MF34G-5 | 34 ga. Filling needle, 67 mm long |
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Cite This Article
Van Hook, M. J., Thoreson, W. B. Simultaneous Whole-cell Recordings from Photoreceptors and Second-order Neurons in an Amphibian Retinal Slice Preparation. J. Vis. Exp. (76), e50007, doi:10.3791/50007 (2013).