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Medicine

Trabajar con Tejidos Humanos para la Investigación del Cáncer Traslacional

Published: November 26, 2015 doi: 10.3791/53189
* These authors contributed equally

Materials

Name Company Catalog Number Comments
Needles BD 305167
Stainless steel disposable scalpels Miltex 4-410
Tissue culture dish Corning 353003
Biopsy Cassettes Thermo Fisher 58931
RNA-stabilizing solution Ambion AM7021
1.5 ml Eppendorf tubes Phenix MAX-815
50 ml tubes Corning 430290
10% Neutral Buffered Formalin StatLab Medical Products 28600-5
Formalin Containers Fisher Scientific 23-032-059
Optimal cutting temperature solution (OCT) Sakura Finetek 4583
Tissue-Tek Cryomold Sakura Finetek 4557
Dnase I Roche 10104159001
Collagenase I Roche 11088793001
70μm cell strainers BD Bioscience 352350
96 well round bottom plates Corning 3799
Live/Dead stain solution Life Technologies L34957

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References

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  2. van der Worp, H. B., et al. Can animal models of disease reliably inform human studies. , PLoS Med. 7, e1000245 (2010).
  3. McCabe, P. M., et al. Animal models of disease. Physiol. Behav. 68, 501-507 (2000).
  4. Woolf, S. H. The meaning of translational research and why it matters. JAMA. 299, 211-213 (2008).
  5. Brockbank, K. G., Taylor, M. J. Tissue Preservation. Advances in Biopreservation. , 157-159 (2006).
  6. Bulmer, M. The ethics of social research. Researching social life. Gilbert, N. , Sage. London. 45-57 (2001).
  7. Penslar, R. L. IRB guidebook. , US Government Printing Office. (1993).
  8. Protection of human subjects. CFR Title 21 Section 50.25. , Food and Drug Administration. Available from: http://www.accessdata.fda.gov/scripts/cdrh/cfdocs/cfcfr/CFRSearch.cfm?FR=50.25 (2011).
  9. HIPAA privacy rule and public health. Guidance from CDC and the US Department of Health and Human Services. , Centers for Disease Control and Prevention. Available from: http://www.cdc.gov/mmwr/preview/mmwrhtml/su5201a1.htm (2003).
  10. MD Anderson Cancer Center Institutional Tissue Bank Standard Operating Procedures. 3, Institutional Tissue Bank. (2009).
  11. Collection, Storage, Retrieval and Distribution of Biological Materials for Research. , 3rd ed., International Society for Biological and Environmental Repositories. Available from: http://biorepository.uic.edu/Contact_Us_files/ISBERBestPractices3rdedition.pdf (2011).
  12. NCI Best Practices for Biospecimen Resources. , 2nd ed., Office of Biorepositories and Biospecimen Research. Available from: http://biospecimens.cancer.gov/bestpractices/2011-NCIbestpractices.pdf (2011).
  13. Ruffell, B., et al. Leukocyte composition of human breast cancer. Proc. of the Ntnl. Aca. of Sci. 109, 2796-2801 (2012).
  14. Shapiro, H. M. Practical flow cytometry. , John Wiley & Sons. (2005).
  15. Baker, A. F., et al. Stability of phosphoprotein as a biological marker of tumor signaling. Clin. Cancer Res. 11, 4338-4340 (2005).
  16. Jacobs, S. Sample processing considerations for detecting copy number changes in formalin-fixed, paraffin-embedded tissues. Cold Spring Harbor protocols. 2012, 1195-1202 (2012).
  17. Chung, J. -Y., et al. Factors in tissue handling and processing that impact RNA obtained from formalin-fixed, paraffin-embedded tissue. J. Histochem. Cytochem. 56, 1033-1042 (2008).
  18. Guo, H., et al. An efficient procedure for protein extraction from formalin-fixed, paraffin-embedded tissues for reverse phase protein arrays. Proteome Sci. 10, 1477-5956 (2012).
  19. Feldman, A. L., et al. Advantages of mRNA amplification for microarray analysis. Biotechniques. 33, 906-914 (2002).
  20. Francino, O., et al. Advantages of real-time PCR assay for diagnosis and monitoring of canine leishmaniosis. Vet Parasitol. 137, 214-221 (2006).
  21. Grunberg-Manago, M. Messenger RNA stability and its role in control of gene expression in bacteria and phages. Annu. Rev. Genet. 33, 193-227 (1999).
  22. Lesnik, E. A., Freier, S. M. Relative thermodynamic stability of DNA, RNA, and DNA:RNA hybrid duplexes: relationship with base composition and structure. Biochemistry. 34, 10807-10815 (1995).
  23. MacBeath, G. Protein microarrays and proteomics. Nat Genet. 32, 526-532 (2002).
Trabajar con Tejidos Humanos para la Investigación del Cáncer Traslacional
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Cite this Article

Reuben, A., Gopalakrishnan, V.,More

Reuben, A., Gopalakrishnan, V., Wagner, H. E., Spencer, C. N., Austin-Breneman, J., Jiang, H., Cooper, Z. A., Wargo, J. A. Working with Human Tissues for Translational Cancer Research. J. Vis. Exp. (105), e53189, doi:10.3791/53189 (2015).

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