The authors have nothing to disclose.
Silicon Wafer | University Wafer | 452 | |
SU-8 2035 | MicroChem | Y111070-0500L1GL | |
Developer | MicroChem | Y020100-4000L1PE | |
Wafer Mask | Cad/Art Services | – | Custom order. Printed at 25,000 dpi. |
Sylgard-184 | Ellsworth Adhesives | 184 SIL ELAST KIT 0.5KG | |
1.0 mm Dermal Punches | Acuderm Inc. | P150 | |
Soft Tubing | Cole-Palmer | EW-06419-01 | |
Hard Tubing | IDEX Health & Science | 1622 | |
Pins | New England Small Tube | NE-1027-12 | |
Blocking Pins | New England Small Tube | 0.415/0.425" OD x .500 Long | Batch PB07027 |
3 mL syringes | BD | 309657 | |
30 mL syringes | Vitality Medical | 302832 | Used as buffer reservoirs. |
Stainless Steel Blunt Needle 23 Gauge, Polyprolylene Luer | Component Supply Company | NE-231PL-50 | |
Stopcocks with Luer connections; 3-way; male lock; 5 flow pattern; non-sterile | Cole-Palmer | EW-30600-07 | |
Fisherfinest Premium Cover Glass | Fisher Scientific | 12-548-5M | |
Mercator Control System LF-5 Plasma System | Mercator | LF-5 | |
Scotch Tape | Scotch | BSN43575 | |
Series 20 Chamber | Warner Instruments | P-2 | |
Vacuum Desicator | Bel-Art Scienceware | 420250000 | 24 cm inner diameter. |
Weigh Boats | Cole-Palmer | EW-01017-27 | |
Classic Plus Balance | Mettler Toledo | PB1501-S/FACT | |
Glass Pasteur Pipettes | Cole-Palmer | EW-25554-06 | |
Transfer pipettes | Genesee Scientific | 30-202 | |
Oven | Sheldon Manufacturing Inc | 9120993 | Model Number: 1500E. |
60 mm, non-vented, sharp edge Petri dishes | TriTech Research | T3308 | |
Zeiss Axio Observer.A1 | Zeiss | – | |
Hammamatsu Orca Flash 4.0 Digital CMOS | Hammamatsu | C11440-22CU | |
Blue Fluorescent Light | Lumencor | SOLA SM6-LCR-SA | 24-30V/7.9A DC. |
Illumination Adaptor | Zeiss | 423302-0000 | |
Series 1 and 2 Miniature Inert PTFE Isolation Valve | Parker | 001-0017-900 | 3-way valve for controlling flow. |
ValveLink8.2® | AutoMate Scientific | 01-18 | Flow Switch Controller |
Micro Manager | Micro-Manager | – | Free software, can be downloaded at: https://www.micro-manager.org/wiki/Download_Micro-Manager_Latest_Release |
ImageJ | ImageJ | – | Free software, can be downloaded at: https://imagej.nih.gov/ij/download.html |
Agar, Bacteriological Grade | Apex | 9012-36-6 | |
Peptone | Apex | 20-260 | |
CaCl<sub>2</sub> | VWR | BDH0224-1KG | |
MgSO<sub>4</sub> | Sigma-Aldrich | 230391-1kg | |
Cholesterol | Alfa Aesar | A11470 | |
Ethanol | Sigma-Aldrich | 270741-4L | |
Tetramisole | Sigma-Aldrich | L9756-10(G) | Store at 4 °C. |
Fluorescein | Sigma-Aldrich | FD2000S-250mg | Light Sensitive. Store in photoprotective vials. |
Glycerol | Sigma-Aldrich | G6279-1L | |
Ascaroside #3 | – | – | Synthesized in the Schroeder Lab (Cornell University). |
NaCl | Genesee Scientific | 18-215 | |
KH<sub>2</sub>PO<sub>4</sub> | BDH | BDH9268.25 | |
K<sub>2</sub>HPO<sub>4</sub> | J.T. Baker | 3252-025 | |
ASH GCaMP3 line | – | – | CX10979 (KyEx2865 [p<em>sra</em>-6::GCAMP3 @ 100 ng/uL]). Developed in Bargmann lab. Provided from Albrecht Lab library. |
CEM GCaMP6 line | – | – | JSR49 (FkEx98[p<em>pkd</em>-2::GCaMP::SL2::dsRED + pBX-1]; <em>pha</em>-1(e2123ts); <em>him</em>-5(e1490); <em>lite</em>-1(ce314)). Developed by Robyn Lints. Provided from Srinivasan Lab library. |
<em>E. coli</em> (OP50) | <em>Caenorhabditis</em> Genetics Center | OP50 | |
"Reservoir" | – | – | To create a Reservoir: A "30 mL syringe", is connected to a "Stopcock with Luer connections; 3-way; male lock; 5 flow pattern; non-sterile", which is connected to a "3 mL syringe" and a "Stainless Steel Blunt Needle 23 Gauge, Polyprolylene Luer". The "Stainless Steel Blunt Needle 23 Gauge, Polyprolylene Luer" is then inserted into "Soft Tubing" approximately 1/3 of the way down the needle. |