Erratum: Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development

Zusammenfassung

An erratum was issued for Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development.

The human RCTEC/RCTE cell stocks used here and in Caralt et al., 2015, Uzarski et al., 2015 were originally provided by Dr. Loghman-Adham (then at St. Louis University) to co-author Dr. Wandinger-Ness (University of New Mexico) through an MTA in 2001. The SV40 immortalized human RCTEC/RCTE cells were characterized as being of distal tubule cell line as detailed in Loghman-Adham et al., 2003. On the basis of recent short tandem repeat (STR) DNA sequencing (Performed by IDEXX BioResearch) of the earliest passages of the RCTEC/RCTE cell stocks it became evident the cells were of mixed lineage. Further analyses of PCR products using QIAxcel capillary electrophoresis demonstrated the presence of a canine product. The product was sequenced and established to be of canine origin. A set of canine specific STR markers were compared to the sample and showed that the sample had a genetic profile with 92% identity to the MDCK cell line.  Later cell stocks that were used in the present publication were STR profiled and showed drift to 100% MDCK lineage. Despite being of canine origin, rather than human as was previously thought, MDCK is similarly a distal tubule epithelial cell line. For this reason, previous interpretation and conclusions drawn using these cells here and in Caralt et al., 2015, Uzarski et al., 2015 remain sound, but for purposes of rigor, reproducibility and experimental validation by others we report on this misidentification. This information will also be reported for listing on the International Cell Line Authentication Committee (ICLAC) database (http://iclac.org).

Zusammenfassung

An erratum was issued for Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development.

The human RCTEC/RCTE cell stocks used here and in Caralt et al., 2015, Uzarski et al., 2015 were originally provided by Dr. Loghman-Adham (then at St. Louis University) to co-author Dr. Wandinger-Ness (University of New Mexico) through an MTA in 2001. The SV40 immortalized human RCTEC/RCTE cells were characterized as being of distal tubule cell line as detailed in Loghman-Adham et al., 2003. On the basis of recent short tandem repeat (STR) DNA sequencing (Performed by IDEXX BioResearch) of the earliest passages of the RCTEC/RCTE cell stocks it became evident the cells were of mixed lineage. Further analyses of PCR products using QIAxcel capillary electrophoresis demonstrated the presence of a canine product. The product was sequenced and established to be of canine origin. A set of canine specific STR markers were compared to the sample and showed that the sample had a genetic profile with 92% identity to the MDCK cell line.  Later cell stocks that were used in the present publication were STR profiled and showed drift to 100% MDCK lineage. Despite being of canine origin, rather than human as was previously thought, MDCK is similarly a distal tubule epithelial cell line. For this reason, previous interpretation and conclusions drawn using these cells here and in Caralt et al., 2015, Uzarski et al., 2015 remain sound, but for purposes of rigor, reproducibility and experimental validation by others we report on this misidentification. This information will also be reported for listing on the International Cell Line Authentication Committee (ICLAC) database (http://iclac.org).

Protokoll

Zu Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development wurde ein Erratum veröffentlicht.

Die hier und in Caralt et al., 2015, Uzarski et al., 2015 verwendeten menschlichen RCTEC/RCTE-Zellbestände wurden ursprünglich im Jahr 2001 von Dr. Loghman-Adham (damals an der St. Louis University) an die Ko-Autorin Dr. Wandinger-Ness (University of New Mexico) im Rahmen einer MTA übergeben. Die mit SV40 immortalisierten menschlichen RCTEC/RCTE-Zellen wurden als Zelllinie des distalen Tubulus charakterisiert, wie ausführlich in Loghman-Adham et al., 2003 beschrieben. Aufgrund aktueller Analysen der kurzen tandemartigen Wiederholungen (STR) mittels DNA-Sequenzierung (durchgeführt von IDEXX BioResearch) der frühesten Passagen der RCTEC/RCTE-Zellbestände zeigte sich, dass die Zellen gemischter Herkunft waren. Weitere Analysen der PCR-Produkte mittels kapillarer Elektrophorese mit QIAxcel ergaben das Vorhandensein eines caninen Produkts. Das Produkt wurde sequenziert und als caninen Ursprungs identifiziert. Ein Satz spezifischer STR-Marker für Hunde wurde mit der Probe verglichen und zeigte, dass die Probe ein genetisches Profil mit 92 % Übereinstimmung mit der MDCK-Zelllinie aufwies.  Spätere Zellbestände, die in der vorliegenden Publikation verwendet wurden, wurden hinsichtlich ihres STR-Profils analysiert und zeigten eine Abweichung hin zu 100 % MDCK-Herkunft. Obwohl die Zellen nicht menschlichen, sondern caninen Ursprungs sind, wie ursprünglich angenommen, stellt MDCK ebenfalls eine epitheliale Zelllinie des distalen Tubulus dar. Aus diesem Grund bleiben die früheren Interpretationen und Schlussfolgerungen, die unter Verwendung dieser Zellen hier sowie in Caralt et al., 2015, Uzarski et al., 2015 gezogen wurden, gültig. Dennoch berichten wir aus Gründen der wissenschaftlichen Strenge, Reproduzierbarkeit und experimentellen Überprüfbarkeit durch Dritte über diese fehlerhafte Identifizierung. Diese Information wird auch für die Aufnahme in die Datenbank des Internationalen Komitees zur Authentifizierung von Zelllinien (International Cell Line Authentication Committee, ICLAC) gemeldet (http://iclac.org).