Crude Plant Extract
Crude Plant Extract - Related Videos
03:58
Preparation of Crude Cell Extract from Vibrio natriegens
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2025
Source: Wiegand, D. J., et al. Cell-free Protein Expression Using the Rapidly Growing Bacterium Vibrio natriegens. J. Vis. Exp. (2019).This video demonstrates the preparation of a cell extract from Vibrio natriegens, a rapidly growing bacterium known for its high protein synthesis rate. The bacterial pellet is washed and resuspended in lysis buffer before sonication on ice to prevent cellular degradation. The lysate is centrifuged to separate insoluble cell debris, and the cell extract is...
03:32
Cell-Free Protein Expression Using a Bacterial Crude Cell Extract
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2025
Source: Wiegand, D. J., et al. Cell-free Protein Expression Using the Rapidly Growing Bacterium Vibrio natriegens. J. Vis. Exp. (2019).This video demonstrates the use of a bacterial cell-free protein expression system based on Vibrio natriegens crude lysate to perform coupled in vitro transcription and translation. It highlights the preparation of a master mix with essential components and the step-by-step synthesis of functional proteins from a DNA template.
08:56
Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
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2018
Here we present a new approach to identify plant viruses with double-strand DNA genomes. We use standard methods to extract DNA and RNA from infected leaves and carry out next-generation sequencing. Bioinformatic tools assemble sequences into contigs, identify contigs representing virus genomes and assign genomes to taxonomic groups.
10:54
Extraction of Plant-based Capsules for Microencapsulation Applications
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Cited by 5 •
2016
This protocol/manuscript describes a streamlined process for the production of sporopollenin exine capsules (SECs) from Lycopodium clavatum spores and for the loading of hydrophilic compounds into these SECs.
03:16
Phospholipase Activity Assay: A Gel Diffusion Assay to Determine Phospholipase Activity in Crude Venom Extract from Anthopleura dowii
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2025
In this video, we demonstrate an assay to establish the presence of phospholipase in the crude venom extract from Anthopleura dowii—a marine sea anemone. The assay utilizes an agarose gel diffusion technique, in which the enzyme phospholipase diffuses and breaks down phospholipids incorporated in the solid media in a radial manner.
FAQs
The solvent or buffer determines which constituents from disrupted tissue enter the liquid phase. The resulting sample may contain different proportions of soluble metabolites, proteins, pigments, lipids, and other cellular components. Consequently, preparations from the same plant material can support different biochemical analyses, and the treatment should be selected according to the intended assay or screening objective.
Multiple constituents can contribute to an observed activity or experimental signal, making it difficult to identify the responsible compound or component. Pigments, lipids, proteins, and metabolites may coexist in the same preparation rather than being measured separately. This uncertainty is especially important when interpreting enzyme assays or bioactive effects, because an apparent result may not have a single identified source.
A crude preparation retains many soluble constituents together, whereas purification or fractionation separates the mixture into more defined portions. The less complex samples can make chemical characterization and attribution of an observed effect more reliable. Researchers therefore often treat the crude material as a starting sample, then use additional separation when they need to associate activity with particular compounds or fractions.
Preparation begins by disrupting plant tissue to release cellular contents. The material is then treated with a selected solvent or buffer so that relevant constituents dissolve into the liquid phase. Filtration or centrifugation removes insoluble material, producing a clarified sample for assays, screening, or profiling. Further purification or fractionation may follow if the mixture remains too complex for confident interpretation.
Both operations remove insoluble material from the liquid portion produced during extraction. Filtration separates the extract by passing it through a barrier, while centrifugation separates components through spinning so insoluble material can be removed from the liquid phase. This clarification makes the preparation more suitable for downstream biochemical measurements without claiming that the remaining solution contains only one class of compound.
They provide starting material for enzyme activity assays, phytochemical screening, metabolite profiling, and investigations of bioactive compounds. These analyses can reveal that plant tissue contains measurable biochemical activities or chemically detectable constituents, but the mixed composition limits direct attribution. Researchers may therefore use purification, fractionation, and chemical characterization to connect an observed outcome with particular components.