High Performance Tlc
High Performance Tlc - Related Videos
12:04
Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds
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Cited by 52 •
2014
Methods are described for thin-layer chromatographic (TLC) separation of plant extracts and contact bioautography to identify antibacterial metabolites. The methods are applied to the screening of red clover phenolic compounds inhibiting hyper ammonia-producing bacteria (HAB) native to the bovine rumen.
01:11
Thin-Layer Chromatography (TLC): Overview
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2025
Thin-layer chromatography (TLC) is a chromatography technique that separates compounds based on their polarity. TLC typically uses polar silica gel, a form of silicon dioxide, as the stationary phase. The silica gel contains hydroxyl (OH) groups on its surface, which form hydrogen bonds with polar compounds, influencing their adhesion to the stationary phase. To begin the analysis, a mixture of compounds is spotted on the starting line on the TLC plate using a thin capillary. The bottom of the...
13:02
Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography (TLC) Coupled with Gas-Liquid Chromatography (GLC)
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Cited by 102 •
2011
Composition of polar lipid extracts and the fatty acid composition of individual glycerolipids are determined in a simple and robust lipid profiling experiment. For this purpose, glycerolipids are isolated by thin layer chromatography and subjected to transmethylation of their acyl groups. Fatty acyl methylesters are quantified by gas-liquid chromatography.
09:48
Performing 1D Thin Layer Chromatography
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2023
Source: Laboratory of Dr. Yuri Bolshan — University of Ontario Institute of Technology Thin layer chromatography (TLC) is a chromatographic method used to separate mixtures of non-volatile compounds. A TLC plate consists of a thin layer of adsorbent material (the stationary phase) fixed to an appropriate solid support such as plastic, aluminum, or glass1. The sample(s) and reference compound(s) are dissolved in an appropriate solvent and applied near the bottom edge of the TLC plate in small...
02:23
Performing Thin-Layer Chromatography to Assess Bacterial Exopolysaccharide Degradation
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2026
Source: Hu, Y., et al. Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems. J. Vis. Exp. (2019)This video demonstrates the use of thin-layer chromatography (TLC) to assess the degradation of exopolysaccharides (EPS) by human fecal microbiota. EPS fragments in fermentation supernatants are separated based on size and polarity using TLC, then visualized as colored bands following carbohydrate-specific staining.
FAQs
HPTLC improves separation quality through finely sized sorbent particles, precise sample application, and controlled solvent development. These features help produce narrow bands with clearer spacing between compounds, which supports more reproducible comparisons and measurements. The improved resolution is especially useful when biochemical samples contain several amino acids, lipids, metabolites, or other compounds that must be distinguished on one plate.
Both factors directly influence band sharpness and reproducibility. Finely sized sorbent particles support improved resolution, while precise application places samples consistently on the plate before solvent development. Together, these design features reduce variation in band position and appearance, making results easier to compare across complex biochemical samples and more suitable for routine analytical workflows.
Visible and ultraviolet detection allow separated analytes to be located through their observed responses after development, whereas densitometric detection supports measurement of those responses. The choice affects whether the analysis emphasizes visual comparison or quantitative assessment. In biochemistry, these detection options help evaluate compound patterns and measure changes associated with purification or reaction monitoring.
An HPTLC workflow begins with precise application of samples to a coated plate, followed by controlled solvent development to separate the compounds. After development, the resulting bands are examined using visible or ultraviolet detection, or measured densitometrically. This sequence converts a complex sample into a resolved pattern that can be compared or evaluated quantitatively.
HPTLC is useful when researchers need to examine amino acids, lipids, metabolites, or other biomolecules across complex samples. Its parallel processing allows multiple samples to be compared in the same analytical setting, while the resolved bands help reveal differences in composition. It can also support monitoring of purification progress or biochemical reaction outcomes.
The method combines parallel processing with relatively low solvent use, allowing many samples to be handled efficiently while limiting solvent requirements. Its improved reproducibility and selectable detection approaches support consistent comparison and measurement. These features make HPTLC valuable for screening, routine biochemical analysis, and repeated checks of sample composition during purification or reaction studies.