Inguinal Adipose Tissue
Inguinal Adipose Tissue - Related Videos
07:59
Measurement of Basal and Forskolin-stimulated Lipolysis in Inguinal Adipose Fat Pads
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Cited by 10 •
2017
This protocol describes the method of determining basal and forskolin-stimulated lipolysis in inguinal fat pads obtained from normal chow diet (NCD) or high fat diet (HFD) ± capsaicin fed wild type mice. As an index for lipolysis, glycerol release was measured from inguinal adipose fat pads.
03:28
White and Brown Adipose Depot Collection from Mouse Pup: A Surgical Procedure to Harvest the White Adipose Tissue and Brown Adipose Tissue from Mouse Pup
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2025
In this video, we demonstrate the surgical procedure to isolate the subcutaneous white adipose tissue and interscapular brown adipose tissue from a newborn mouse.
07:34
Intravital Microscopy of the Inguinal Lymph Node
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Cited by 12 •
2011
A technique for performing intravital microscopy of the inguinal lymph node (LN) is outlined. Such technique allows for real-time, in vivo study of the lymph node microvasculature and structure both during homeostasis and infection. This technique can be adapted to cell trafficking studies and to other lymph node sites.
10:14
Inguinal Subcutaneous White Adipose Tissue (ISWAT) Transplantation Model of Murine Islets
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Cited by 4 •
2020
In this protocol, a method of murine islet isolation and transplantation into the inguinal subcutaneous white adipose tissue is described. Isolated syngeneic murine islets are transplanted into a murine recipient using a basement membrane hydrogel. The blood glucose level of the recipients is monitored, and histology analysis of the islet grafts is performed.
07:09
Isolation of Adipose Tissue Immune Cells
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Cited by 75 •
2013
Adipose tissue (AT) is a site of intense immune cell activation and interaction. Almost all cells of the immune system are present in AT and their ratios are altered by obesity. Proper isolation, quantification, and characterization of AT immune cell populations are critical for understanding their role in immunometabolic disease.
FAQs
Within adipocytes, triglycerides are retained in lipid droplets until regulatory signals activate lipolysis, the breakdown process that releases fatty acids. Hormonal and sympathetic inputs therefore shift the depot between storage and mobilization. Studying this switch helps biochemists connect intracellular lipid handling with energy balance and assess how signaling changes may influence metabolic regulation.
Cold exposure and β-adrenergic stimulation can promote a white-to-beige adipocyte transition in this depot. Beige cells acquire thermogenic behavior through mitochondrial uncoupling protein 1 activity, linking mitochondrial function with thermogenic output rather than focusing solely on lipid storage. This makes the tissue useful for examining how environmental or receptor-mediated cues alter energy expenditure.
These are distinct processes: lipolysis mobilizes stored triglycerides and releases fatty acids, whereas beige conversion changes adipocyte thermogenic properties through UCP1-associated mitochondrial uncoupling. Considering them separately helps researchers determine whether a stimulus primarily affects fuel availability, thermogenic programming, or both. The distinction is important when interpreting metabolic responses in biochemical experiments.
Because the depot supports both lipid storage and thermogenic remodeling, it provides a setting for studying differentiation rather than examining mature storage alone. Investigators can relate adipocyte state to triglyceride handling, lipolysis, and UCP1-linked mitochondrial uncoupling. This connects cell development with energy balance and offers biochemical context for how fat-cell properties change.
Insulin sensitivity and obesity research benefit from examining this depot because several linked processes can be considered together: triglyceride storage, fatty-acid release through lipolysis, thermogenic beige conversion, and obesity-related tissue remodeling. This integrated view helps connect adipocyte biochemistry with whole-body energy balance and metabolic regulation, while preserving the distinction between storage and thermogenic functions.
Comparative studies can ask whether hormonal or sympathetic signals favor fatty-acid mobilization, whether cold or β-adrenergic stimulation is associated with beige-cell conversion, and how these responses relate to energy balance. Examining those outcomes in the same depot helps researchers organize metabolic effects into storage, release, thermogenesis, and remodeling categories, providing a structured context for biochemistry experiments.