Inguinal Subcutaneous White Adipose Tissue (ISWAT) Transplantation Model of Murine Islets

Yuanzheng Peng; Zhicheng Zou; Jiao Chen; Hancheng Zhang; Ying Lu; Rito Bittino; Hongxing Fu; David K. C. Cooper; Shan Lin; Mengtao Cao; Yifan Dai; Zhiming Cai; Lisha Mou

doi:10.3791/60679

Journal

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Medicine

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Inguinal Subcutaneous White Adipose Tissue (ISWAT) Transplantation Model of Murine Islets

JoVE Journal
Medicine

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JoVE Journal Medicine

Inguinal Subcutaneous White Adipose Tissue (ISWAT) Transplantation Model of Murine Islets

6,386 Views

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10:14 min

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February 16, 2020

DOI:

10.3791/60679-v

DOI:

10.3791/60679-v

•

10:14 min

February 16, 2020

•

6368 Views

Yuanzheng Peng*¹, Zhicheng Zou*¹, Jiao Chen¹, Hancheng Zhang¹, Ying Lu¹, Rito Bittino², Hongxing Fu³, David K. C. Cooper⁴, Shan Lin¹, Mengtao Cao¹, Yifan Dai⁵, Zhiming Cai¹, Lisha Mou¹

¹Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen Second People’s Hospital,First Affiliated Hospital of Shenzhen University, Health Science Center ²Institute for Cellular Therapeutics,Allegheny-Singer Research Institute ³School of Pharmaceutical Sciences,Wenzhou Medical University ⁴Xenotransplantation Program/Department of Surgery,University of Alabama at Birmingham ⁵Jiangsu Key Laboratory of Xenotransplantation,Nanjing Medical University

Summary

In this protocol, a method of murine islet isolation and transplantation into the inguinal subcutaneous white adipose tissue is described. Isolated syngeneic murine islets are transplanted into a murine recipient using a basement membrane hydrogel. The blood glucose level of the recipients is monitored, and histology analysis of the islet grafts is performed.