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Inguinal Subcutaneous White Adipose Tissue (ISWAT) Transplantation Model of Murine Islets
Inguinal Subcutaneous White Adipose Tissue (ISWAT) Transplantation Model of Murine Islets
JoVE Journal
Medicine
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JoVE Journal Medicine
Inguinal Subcutaneous White Adipose Tissue (ISWAT) Transplantation Model of Murine Islets

Inguinal Subcutaneous White Adipose Tissue (ISWAT) Transplantation Model of Murine Islets

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10:14 min

February 16, 2020

DOI:

10:14 min
February 16, 2020

6368 Views
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Summary

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In this protocol, a method of murine islet isolation and transplantation into the inguinal subcutaneous white adipose tissue is described. Isolated syngeneic murine islets are transplanted into a murine recipient using a basement membrane hydrogel. The blood glucose level of the recipients is monitored, and histology analysis of the islet grafts is performed.

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