Bone Construct Fabrication
Bone Construct Fabrication - Related Videos
13:43
Fabrication of Myogenic Engineered Tissue Constructs
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Cited by 11 •
2009
Here, we demonstrate fabrication of collagen-based, tissue constructs containing skeletal myoblasts. These 3-D engineered constructs may be used to replace or repair tissues in vivo. For our purposes, we have designed these as an atrioventricular electrical conduit for the repair of complete heart block[1].
08:49
Models of Bone Metastasis
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Cited by 115 •
2012
Animal models are frequently utilized to study cancer metastasis to bone. In this protocol we will describe two common methods of tumor inoculation for bone metastasis studies and briefly describe some of the analyses utilized to monitor and quantify these models.
13:48
Design and Construction of an Urban Runoff Research Facility
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Cited by 6 •
2014
This paper describes the design, construction, and function of a 1,000 m2 facility containing 24 individual 33.6 m2 field plots equipped for measuring total runoff volumes with time and collection of runoff subsamples at selected intervals for quantification of chemical constituents in the runoff water from simulated home lawns.
11:11
Construction and Characterization of a Novel Vocal Fold Bioreactor
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Cited by 8 •
2014
A novel vocal fold bioreactor capable of delivering physiologically relevant, vibratory stimulation to cultured cells is constructed and characterized. This dynamic culture device, when combined with a fibrous poly(ε-caprolactone) scaffold, creates a vocal fold-mimetic environment that modulates the behaviors of mesenchymal stem cells.
07:53
Using Adhesive Patterning to Construct 3D Paper Microfluidic Devices
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Cited by 4 •
2016
We demonstrate the use of patterned aerosol adhesives to construct 3D paper microfluidic devices. This method of adhesive application forms semi-permanent bonds between layers, enabling single-use devices to be non-destructively disassembled after use and to ease folding complex nonplanar structures.
FAQs
Pore architecture helps determine how nutrients move through the construct and how cells interact with its three-dimensional environment. Designing interconnected porous structures can support nutrient transport while also influencing mechanical properties, cell attachment, and mineral deposition. These trade-offs matter because a construct must provide a suitable environment for tissue development without losing the structural characteristics needed for repair.
Biochemical and mechanical cues guide cellular behavior after the construct is formed. They can promote cell attachment and proliferation, encourage osteogenic differentiation, and influence the deposition of mineral. Combining these signals helps researchers move beyond simply providing a scaffold, creating an environment that directs bone-forming activity and supports the development of tissue with more relevant biological characteristics.
These fabrication approaches provide different ways to control the architecture of a bone construct. 3D printing, molding, and bioprinting can each be used to shape three-dimensional structures, while the selected method affects how researchers organize porous features, biomaterials, and cells. This design control allows constructs to be tailored for nutrient transport, mechanical properties, and biological guidance.
A typical workflow begins by selecting biomaterials and a structural format, then establishing porous architecture through methods such as 3D printing, molding, or bioprinting. Bone-forming cells and relevant biochemical or mechanical cues can be incorporated to support attachment, proliferation, and osteogenic differentiation. Researchers then focus on how the resulting design supports mineral deposition and tissue development.
Researchers use these constructs as platforms for studying bone development and testing therapies, as well as for addressing defects caused by trauma, disease, or surgery. Their controlled three-dimensional organization makes it possible to examine how biomaterials, cells, architecture, and environmental cues contribute to bone-forming responses, supporting both experimental investigation and the development of regenerative strategies.
Important challenges include achieving adequate vascularization, promoting integration with surrounding tissue, and maintaining functional performance over time. These issues influence whether a construct can support tissue development and remain effective after implantation. Current advances therefore focus not only on forming bone-like architecture and mineralized tissue, but also on improving the biological connection and durability of the resulting structure.