Chemokine Sdf-1 Alpha
Chemokine Sdf-1 Alpha - Related Videos
12:00
Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles
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Cited by 9 •
2015
The objective of this protocol is to incorporate SDF-1α, a stem cell homing factor, into dextran sulfate-chitosan nanoparticles. The resultant particles are measured for their size and zeta potential, as well as the content, activity, and in vitro release rate of SDF-1α from the nanoparticles.
10:56
A Novel Three-dimensional Flow Chamber Device to Study Chemokine-directed Extravasation of Cells Circulating under Physiological Flow Conditions
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Cited by 7 •
2013
The three-dimensional flow chamber device is a novel in vitro technology for the quantitative and step-wise evaluation of the extravasation cascade of cells circulating under conditions of physiological shear stress. The device therefore fills a critical need for basic, preclinical, and clinical studies of cell migration.
03:25
Immunostaining of Neurons Treated with Alpha-Synuclein Aggregates
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2025
This video demonstrates the immunostaining of neurons treated with alpha-synuclein (αS) aggregates. The neurons are first treated with αS aggregates. They are then fixed, permeabilized, and treated with a blocking solution. Primary antibodies are added, followed by a fluorescently labeled secondary antibodies and a nuclear stain. The sample is then mounted and observed under a microscope.
01:30
Detection of Phosphorylated Alpha-Synuclein Using Western Blotting
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2025
Add a loading buffer containing a detergent, a reducing agent, and a tracking dye.
02:12
Induction of Neuroinflammation in Mice via Injection of Alpha-Synuclein Fibrils
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2025
This video demonstrates the injection of alpha-synuclein fibrils into a mouse to induce neuroinflammation. The fibrils are injected intraperitoneally into a transgenic mouse expressing mutated alpha-synuclein proteins. Upon reaching the central nervous system, the fibrils enter neurons and induce aggregation of the mutated proteins. The aggregates spread to neighboring neurons, microglia, and astrocytes. Recognition of the aggregates triggers microglia to induce neuroinflammation, damaging the...
FAQs
Binding of SDF-1 alpha to CXCR4 and CXCR7 activates intracellular signaling pathways rather than serving only as a positional cue. Those signals can guide cell migration while also influencing survival, adhesion, and tissue homing. In engineered systems, receptor engagement therefore connects the delivered cue with coordinated cell movement and retention.
Controlled spatial distribution of SDF-1 alpha can create gradients that provide directional information to responsive cells. Instead of exposing a tissue uniformly, a gradient can help organize where cells move and remain. This principle is relevant when scaffolds are designed to recruit cells toward developing or injured tissue regions.
Engagement of CXCR4 and CXCR7 is associated with several coordinated cellular responses. In addition to directing movement, the resulting signaling can influence cell survival, adhesion, and homing to tissue sites. These combined effects make SDF-1 alpha useful when bioengineered systems must support both cell positioning and persistence during regeneration.
Bioengineering strategies can incorporate SDF-1 alpha into biomaterials, hydrogels, or engineered scaffolds. The material serves as a localized platform for presenting the chemokine to cells rather than relying only on unrestricted distribution. Such incorporation supports designs intended to recruit stem or progenitor cells and regulate their placement within regenerating tissue.
Controlled delivery helps establish and maintain chemotactic guidance within an engineered construct. By managing how SDF-1 alpha is presented in the material, researchers can support gradients that encourage cell recruitment, organization, and retention. This approach is especially relevant when regeneration depends on directing cells through a developing or injured tissue environment.
SDF-1 alpha-based systems are relevant to regenerative medicine and cell-guiding strategies that require targeted recruitment of stem or progenitor cells. Engineered materials can use the chemokine to support vascularization, improve cell organization, and promote tissue repair. These outcomes reflect its role as a controllable biological cue within biomaterials and scaffolds.