Chlorella Vulgaris
Chlorella Vulgaris - Related Videos
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Agrobacterium tumefaciens-Mediated Genetic Engineering of Green Microalgae, Chlorella vulgaris
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Cited by 3 •
2023
This protocol outlines the utilization of Agrobacterium tumefaciens-mediated transformation (AMT) for integrating gene(s) of interest into the nuclear genome of the green microalgae Chlorella vulgaris, leading to the production of stable transformants.
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The Infiltration-centrifugation Technique for Extraction of Apoplastic Fluid from Plant Leaves Using Phaseolus vulgaris as an Example
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Cited by 100 •
2014
This protocol details the optimized extraction of apoplast washing fluid from plant leaves, using French bean plants (Phaseolus vulgaris) as a model example.
08:03
Multi-analyte Biochip (MAB) Based on All-solid-state Ion-selective Electrodes (ASSISE) for Physiological Research
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Cited by 3 •
2013
All-solid-state ion-selective electrodes (ASSISEs) constructed from a conductive polymer (CP) transducer provide several months of functional lifetime in liquid media. Here, we describe the fabrication and calibration process of ASSISEs in a lab-on-a-chip format. The ASSISE is demonstrated to have maintained a near-Nernstian slope profile after prolonged storage in complex biological media.
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Enrichment Cultures: Culturing Aerobic and Anaerobic Microbes on Selective and Differential Medias
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2023
Source: Christopher P. Corbo1, Jonathan F. Blaize1, Elizabeth Suter1 1 Department of Biological Sciences, Wagner College, 1 Campus Road, Staten Island NY, 10301 Prokaryotic cells are able to inhabit nearly every environment on this planet. As a kingdom, they possess a great metabolic diversity, allowing them to use a wide variety of molecules for energy generation (1). Therefore, when cultivating these organisms in the lab, all necessary and specific molecules required to make energy must be...
10:58
Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean
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Cited by 15 •
2017
Promoter expression analyses are crucial to improving the understanding of gene regulation and the spatiotemporal expression of target genes. Herein we present a protocol to identify, isolate, and clone a plant promoter. Further, we describe the characterization of the nodule-specific promoter in the common bean hairy roots.
FAQs
Photosynthesis supplies the central conversion mechanism: C. vulgaris uses light energy to transform carbon dioxide, water, and nutrients into cellular material. This links cultivation inputs directly to biomass formation and gives engineers several variables to control. Adjusting those inputs within a culture system can therefore affect both how much biomass accumulates and which biomolecules become prominent.
Growth conditions influence more than cell quantity. The overview indicates that controlled cultivation can alter biochemical composition, including the relative production of proteins, lipids, pigments, and carbohydrates. Consequently, a bioengineering process can be organized around a desired output, such as biomass itself or a particular class of cellular compound, rather than treating all cultures as chemically identical.
Metabolic flexibility allows the organism to serve different process goals under controlled culture conditions. The same platform can be investigated for biomass production, nutrient recovery, carbon capture, or generation of diverse biomolecules. This adaptability is important in bioengineering because researchers can study how cultivation design connects environmental inputs with useful cellular outputs across several sustainable-processing objectives.
Photobioreactors provide a defined setting for cultivating C. vulgaris while researchers examine how controlled conditions affect growth and biochemical composition. Their relevance lies in connecting biological activity with engineered process design: the system can be studied for biomass generation, carbon capture, nutrient recovery, and compound production. This makes photobioreactor research a bridge between algal biology and scalable bioprocessing.
At a general level, cultivation uses a photobioreactor or another engineered culture system to provide light, carbon dioxide, water, and nutrients under controlled conditions. Researchers can then examine growth together with biochemical composition. This workflow connects resource inputs with outputs such as biomass and proteins, lipids, pigments, or carbohydrates without limiting the process to a single product.
These studies use the alga's growth process as a biological route for examining how carbon dioxide and nutrients are incorporated into cellular material. In engineered culture systems, researchers can investigate biomass production alongside these resource-management goals. The approach is relevant to sustainable bioprocessing because one cultivation platform can address carbon handling, nutrient use, and generation of potentially valuable algal biomass.
Product development can target either whole biomass or selected biomolecule classes produced by the culture. The overview identifies proteins, lipids, pigments, and carbohydrates as relevant outputs, while also emphasizing scalable cultivation. This combination lets researchers connect culture design with product concepts, using biochemical composition as a basis for evaluating which algae-based products a process might support.