Custom Fit Fixtures

Custom fit fixtures are purpose-built supports, mounts, or positioning tools shaped to match the geometry and requirements of a specific bioengineering experiment or device. By conforming to a sample, component, or test setup, they stabilize parts, control alignment and loading, and reduce movement or variation during fabrication, measurement, or testing. These fixtures can improve experimental repeatability while protecting delicate biological materials and interfaces from unintended stress. Their use supports reliable prototyping, device characterization, tissue and biomaterial studies, and other workflows in which standard laboratory holders cannot provide the precision, access, or stability required.

Custom Fit Fixtures - Related Videos

Research

JoVE EoE - Neuropathology

Studying the Permeation of FITC and FITC-Ferritin through an In Vitro Blood-Brain Barrier Model

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2025

This video demonstrates a method to study permeation through an in vitro blood-brain barrier (BBB) model using astrocytes and endothelial cells on a membrane insert, with free FITC and FITC-loaded ferritin. FITC-ferritin bound to receptors on endothelial cells and crossed the membrane barrier to the lower chamber while free FITC showed restricted permeation and remained in the upper chamber.

FITC-Dextran Feeding: A Method to Quantify Intestinal Permeability in C. elegans

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2023

This video introduces a method of to visualize and measure the integrity of the C. elegans intestine lumen by feeding worms FITC-labeled dextran. The example protocol shows the assay done with 3,3’-diindolylmethane (DIM)-treated and pathogen-fed worms.

Education

JoVE Business - Marketing
Free Sample

Customer Lifetime Value

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2024

Customer Lifetime Value (CLV) is a metric that quantifies the total revenue a business expects to earn from a customer throughout their entire relationship. It involves predicting the monetary value a customer will bring over time, factoring in their purchasing patterns, frequency, and duration of engagement. CLV is crucial for businesses to assess the long-term profitability of acquiring and retaining customers. By understanding the value each customer contributes, companies can optimize...

Customer Persona

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2024

A customer persona, or buyer persona, is a semi-fictional representation of an ideal customer derived from market research and actual customer data. It serves as a fundamental tool in marketing and product development. Key points about customer personas include: Detailed Profile: A customer persona goes beyond basic demographics, encompassing needs, preferences, behaviors, motivations, pain points, and daily life. Personalization: Customer personas humanize the target audience by assigning a...

Imaging FITC-dextran as a Reporter for Regulated Exocytosis

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Cited by 10 •

2018

Here we detail a method for live cell imaging of regulated exocytosis. This method utilizes FITC-dextran, which accumulates in lysosome-related organelles, as a reporter. This simple method also allows distinguishing between different modes of regulated exocytosis in cells that are difficult to manipulate genetically.

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