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JoVE Journal
Biology

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Imaging FITC-dextran as a Reporter for Regulated Exocytosis
 

Imaging FITC-dextran as a Reporter for Regulated Exocytosis

Article DOI: 10.3791/57936
June 20th, 2018

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Summary June 20th, 2018

Here we detail a method for live cell imaging of regulated exocytosis. This method utilizes FITC-dextran, which accumulates in lysosome-related organelles, as a reporter. This simple method also allows distinguishing between different modes of regulated exocytosis in cells that are difficult to manipulate genetically.

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