Porous Substrate Electroporation
Porous Substrate Electroporation - Related Videos
08:06
Porous Substrate-Based Electroporation with Transepithelial Electrical Impedance Monitoring
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Cited by 2 •
2024
Porous substrate electroporation (PSEP) pairs consistent, high throughput delivery with high cell viability. Introduction of transepithelial electrical impedance (TEEI) measurements provides insight into the intermediate processes of PSEP and allows for label-free delivery. This article discusses a method for performing PSEP delivery experiments and TEEI measurement analysis simultaneously.
05:02
Procedure for the Transfer of Polymer Films Onto Porous Substrates with Minimized Defects
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Cited by 4 •
2019
We present a procedure for highly controlled and wrinkle-free transfer of block copolymer thin films onto porous support substrates using a 3D-printed drain chamber. The drain chamber design is of general relevance to all procedures involving transfer of macromolecular films onto porous substrates, which is normally done by hand in an irreproducible fashion.
11:57
Electroporation of Mycobacteria
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Cited by 55 •
2008
Mycobacterial pathogenic strategies remain poorly understood. The slow growth rate of most species, the impenetrable nature of the cell-wall, and the hazards of working with pathogens make mycobacteria difficult to study and are largely responsible for our poor understanding of these organisms. In this video we will demonstrate the technique of electroporation, which involves subjecting cells to a brief high electrical impulse to allow the entry of DNA. It is the most widely used method for...
08:05
Murine In Utero Electroporation
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2023
In utero electroporation is an important technique for studying the molecular mechanisms that guide the proliferation, differentiation, migration, and maturation of cells during neural development. Electroporation enables the rapid and targeted delivery of material into cells by utilizing electrical pulses to create transient pores in cell membranes. Although electroporation has traditionally been used in in vitro studies, scientific advancements have now broadened its utilization to intact...
12:19
Bacterial Transformation: Electroporation
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2023
The term “transformation” refers cellular ingestion of foreign DNA. In nature, transformation can occur in certain types of bacteria. In molecular biology, however, transformation is artificially induced through the creation of pores in the bacterial cell walls. Bacterial cells that are able to take up DNA from the environment are called competent cells. Electrocompetent cells can be produced in the laboratory and transformation of these cells can be achieve via the application of an...
FAQs
Short electrical pulses temporarily alter the cell membrane by generating localized electric fields across it. These fields form transient pores, creating openings through which nucleic acids, proteins, or other cargos can pass. When the pulse ends, the membrane reseals, allowing delivery without requiring a permanently disrupted membrane.
The substrate has two roles: its electrical conductivity helps establish the localized field, while its porous structure provides an interface where cells can remain attached during treatment. This combination links physical cell support with molecular delivery, making the approach relevant when researchers want cells to stay organized rather than suspended during transfection.
Compared with conventional electroporation, the porous-substrate format acts at the cell–substrate interface rather than relying only on bulk exposure. Its localized operation may limit how broadly cells and surrounding medium experience the electrical treatment. That distinction is important for bioengineering designs requiring spatially controlled delivery while preserving the attached-cell arrangement.
The cargo need not be limited to one molecular class: the described approach can introduce nucleic acids, proteins, or other cargos. This breadth supports different bioengineering objectives, including gene delivery and cell reprogramming. It is especially relevant for difficult-to-transfect cells, for which achieving effective molecular delivery can be challenging.
A basic workflow begins by allowing cells to adhere to the conductive, porous interface. The selected cargo is then present during application of short electrical pulses, which create membrane pores and permit entry. After pulsing, membrane resealing leaves the treated cells attached to the substrate, supporting subsequent spatially organized manipulation.
In bioengineering, this method is useful when delivery must be combined with control over where cells are treated. Supported uses include spatially controlled gene delivery, cell reprogramming, and manipulation of difficult-to-transfect cells. Because cells remain on the interface, the substrate can also support attachment and organization during these interventions.