Y-shaped Mixing Channel

A Y-shaped mixing channel is a microfluidic device that combines two fluid streams at a branching junction, enabling controlled mixing in small volumes. In bioengineering applications, fluids typically flow under laminar conditions, so mixing occurs primarily through molecular diffusion as the streams merge and travel along the shared channel; channel geometry and flow rate influence the mixing time and efficiency. These devices support rapid preparation of chemical reagents, nanoparticles, cell-compatible solutions, and biological samples while requiring minimal material. Their predictable fluid handling makes them valuable for lab-on-a-chip systems, diagnostics, drug delivery research, and studies of reaction kinetics.

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Education

JoVE Core - Chemistry

Molecular Shape and Polarity

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2020

Dipole Moment of a Molecule Polar covalent bonds connect two atoms with differing electronegativities, leaving one atom with a partial positive charge (δ+) and the other atom with a partial negative charge (δ–), as the electrons are pulled toward the more electronegative atom. This separation of charge gives rise to a bond dipole moment. The magnitude of a bond dipole moment is represented by the Greek letter mu (µ) and is given by the formula shown here, where Q is the magnitude of the...

Research

JoVE Journal - Developmental Biology

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This work describes a protocol for quantifying craniofacial cartilage shape using free software (tpsDigs2, MorphoJ, and PAST) to measure changes in facial structure in zebrafish larvae.

VSEPR Theory and the Basic Shapes

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2020

Overview of VSEPR Theory Valence shell electron-pair repulsion theory (VSEPR theory) enables us to predict the molecular structure, including approximate bond angles around a central atom, of a molecule from an examination of the number of bonds and lone electron pairs in its Lewis structure. The VSEPR model assumes that electron pairs in the valence shell of a central atom will adopt an arrangement that minimizes repulsions between these electron pairs by maximizing the distance between them.

Research

JoVE Journal - Bioengineering
Free Sample

Microfluidic Fabrication of Polymeric and Biohybrid Fibers with Predesigned Size and Shape

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Cited by 12 •

2014

Two adjacent fluids passing through a grooved microfluidic channel can be directed to form a sheath around a prepolymer core; thereby determining both shape and cross-section. Photoinitiated polymerization, such as thiol click chemistry, is well suited for rapidly solidifying the core fluid into a microfiber with predetermined size and shape.

RNA Secondary Structure Prediction Using High-throughput SHAPE

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Cited by 15 •

2013

High-throughput selective 2' hydroxyl acylation analyzed by primer extension (SHAPE) utilizes a novel chemical probing technology, reverse transcription, capillary electrophoresis and secondary structure prediction software to determine the structures of RNAs from several hundred to several thousand nucleotides at single nucleotide resolution.

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