Optical Scanner
Optical Scanner - Related Videos
08:31
Three-dimensional Optical-resolution Photoacoustic Microscopy
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Cited by 27 •
2011
Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging technology capable of imaging optical absorption contrasts in vivo with cellular resolution and sensitivity. Here, we provide a visualized instruction on the experimental protocols of OR-PAM, including system configuration, system alignment, typical in vivo experimental procedures, and functional imaging schemes.
07:01
Frequency Mixing Magnetic Detection Scanner for Imaging Magnetic Particles in Planar Samples
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Cited by 5 •
2016
A scanner for imaging magnetic particles in planar samples was developed using the planar frequency mixing magnetic detection technique. The magnetic intermodulation product response from the nonlinear nonhysteretic magnetization of the particles is recorded upon a two-frequency excitation. It can be used to take 2D images of thin biological samples.
08:25
Using Flatbed Scanners to Collect High-resolution Time-lapsed Images of the Arabidopsis Root Gravitropic Response
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Cited by 6 •
2014
This protocol describes a process for rapid collection of images of Arabidopsis seedlings responding to a gravity stimulus using commercially-available flatbed scanners. The method allows for inexpensive, high-volume capture of high-resolution images amenable for downstream analysis algorithms.
09:39
Optical Biosensing
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2023
Optical biosensors utilize light to detect the binding of a target molecule. These sensors can utilize a label molecule, which produces a measurable signal such as fluorescence, or these sensors can be label-free and use the changes in optical properties, such as refractive index, to sense for the binding of the target molecule. This video introduces both label and label-free optical biosensors, demonstrates their use in the laboratory, and shows some applications of the technology.
13:39
Optical Trapping of Nanoparticles
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Cited by 7 •
2013
The following setup approach details low power optical trapping of dielectric nanoparticles using a double-nanohole in metal film.
FAQs
A focused beam or line illuminates a defined portion of the specimen, and the instrument collects reflected, transmitted, or emitted light with a detector. As the illuminated region is mapped across the sample, the detector output is converted into spatial or spectral information. This produces digital data that can be examined as an image or measurement.
Filters and wavelength selection help distinguish fluorescent signals from background light. By limiting which wavelengths reach the detector, the scanner can separate signal associated with a labeled biological target from unrelated optical information. This improves the interpretability of fluorescence measurements in samples such as cells, tissue sections, gels, and microarrays.
Reflected light returns from the illuminated sample, transmitted light passes through it, and emitted light originates from the specimen after excitation, as with fluorescence. These collection pathways provide different kinds of sample information. Selecting among them allows an optical scanner to capture structural or signal-related data appropriate to the specimen being examined.
Spatial information shows where a feature occurs across a specimen, while spectral information describes optical signals associated with selected wavelengths. Together, these measurements can connect location with signal identity or intensity. In biological techniques, that combination supports examination of labeled cells, tissue sections, gels, and microarrays at image or measurement level.
The sample is positioned for scanning, a focused beam or line illuminates its surface or area, and the instrument collects the resulting reflected, transmitted, or emitted light. Filters or wavelength selection may be applied when fluorescence is being measured. The collected signal is then mapped across the specimen and converted into digital results.
Optical scanners can be applied to fluorescently labeled cells, tissue sections, gels, microarrays, and other physical samples that provide detectable optical information. The same general approach supports both image formation and measurement. This range makes the instrument useful for examining biological structures, locating labeled signals, and comparing optical patterns among samples.
The resulting digital data can support quantitative imaging, molecular detection, sample comparison, and documentation of biological structures or activity. Quantitative imaging extracts measurable optical information, while molecular detection can rely on fluorescently labeled targets. Recorded images and measurements also provide a basis for comparing specimens and preserving experimental observations.