Three-dimensional Optical-resolution Photoacoustic Microscopy

The overall goal of this procedure is to demonstrate in vivo optical resolution photoacoustic microscopy or, or Pam of microcirculation that is non-invasive and label free. This is accomplished by first anesthetizing the experimental animal. The second step of the procedure is to position the region of interest by translating the imaging head of the optical resolution photoacoustic microscope scanning parameters are then set and image acquisition is initiated.

Ultimately, the distributions of total oxygen concentration and oxygen saturation of hemoglobin are obtained through computation of the multi wavelength experimental data. Optical resolution photoacoustic microscopy is based on laser excitation and ultrasonic detection. A short post laser beam is focused into biological tissue.