3d Culture Techniques
3d Culture Techniques - Related Videos
05:18
Selective Cell Elimination from Mixed 3D Culture Using a Near Infrared Photoimmunotherapy Technique
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Cited by 21 •
2016
Eliminating specific cells without damaging other cells is extremely difficult, especially in established tissue, yet there is an urgent need for a cell elimination method in the tissue engineering field. Here, we present a method for specific cell elimination from a mixed 3D cell culture using near infrared photoimmunotherapy (NIR-PIT).
03:40
Generation of Microtumors: An In Vitro Culture Technique to Culture 3D Microtumors to Analyze Response to Drug Treatment
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2025
In this video, we generate microtumors from patient-derived tumor cells using 3D human biogel. These microtumors closely resemble the in vivo tumor microenvironment and serve as potential platforms for drug testing.
04:43
Esophageal 3D Raft Culture Model: An In Vitro Culture Technique to Generate Human Esophageal Mucosa Model
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2023
This video describes an in vitro culture technique to generate human organotypic esophageal constructs by co-culturing primary human esophageal fibroblast and squamous epithelial cells within a decellularized porcine scaffold. These 3D culture models mimic the function and physiology of their in vivo tissue counterparts.
03:53
Laser Microdissection: A Technique to Isolate Invading Colorectal Cancer cells from a 3D Organotypic Culture Model
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2023
The video describes the laser dissection of the 3D organotypic co-culture model with micron-level precision. In 3D organotypic models, stromal constituents such as fibroblasts are 3-dimensionally co-cultured with cancer epithelial cells. The 3D model serves as an experimental tool that enables invasion and cancer-stroma interactions to be studied in near-physiological conditions.
04:41
Magnetically Bioprinted 3D Spheroid Co-cultures: A Method for Co-culturing Cancerous Cells and Fibroblasts
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2023
This video explains the 3D magnetic bioprinting of co-cultured pancreatic cancer cells and fibroblasts. Pancreatic cancer cells and activated pancreatic fibroblast cells are co-cultured and magnetized using a biocompatible nanoparticle assembly, to generate spheroids, under the influence of magnetic forces.
FAQs
Cell–cell interactions and extracellular matrix cues provide spatial information that influences how cells organize, differentiate, and function. In a 3D environment, these signals operate within a tissue-like arrangement rather than across a flat surface. Their combined effects help determine whether cells form organized aggregates, spheroids, organoids, or scaffold-supported structures with distinct biological properties.
These formats represent different ways of arranging cells in three dimensions. Aggregates and spheroids emphasize clustered cell organization, while organoids provide a model for more complex tissue architecture. Scaffold-supported structures add an external framework that can influence spatial arrangement. Selecting among them depends on the biological organization and function the researcher aims to study.
Controlled culture conditions are central because they affect how cells interact, organize, and acquire specialized characteristics. Changes in the surrounding environment can therefore alter tissue-like structure and cellular function. Researchers must relate observed outcomes to the conditions used, since the same cell population may display different organization or differentiation patterns in different 3D settings.
Flat cultures provide limited spatial organization, whereas three-dimensional arrangements allow cells to experience interactions and extracellular matrix cues in a tissue-like context. This added organization can expose differences in cell behavior, differentiation, and function that are not apparent in conventional monolayers. Consequently, 3D systems can offer a more informative view of tissue biology and treatment responses.
Model design should match the biological question, the desired spatial arrangement, and the function being examined. Researchers may choose aggregates, spheroids, organoids, or scaffold-supported structures, then maintain controlled conditions that support the intended organization and differentiation. This alignment helps ensure that the resulting model addresses development, disease, treatment response, or another defined biological process.
In biology, these models support investigations of development, tissue architecture, disease progression, and responses to drugs or other treatments. They also provide platforms for personalized research by representing tissue-related behavior in a controlled setting. In regenerative medicine, their ability to model organized tissue structures makes them relevant for studying how cells form and function within tissue-like environments.