Primordium Unrolling
Primordium Unrolling - Related Videos
06:11
Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal Imaging
0 Views •
Cited by 1 •
2023
Maize leaf primordia are deeply ensheathed and rolled, making them difficult to study. Here, we present methods for preparing transverse sections and unrolled whole mounts of maize leaf primordia for fluorescence and confocal imaging.
05:19
In Utero Electroporation Based Gene Delivery: A Technique to Inject Plasmid DNA into Embryonic Cerebellar Cells of Murine Embryos
0 Views •
2025
This video demonstrates the delivery of plasmid DNA into cerebellar neuronal cells of murine embryos using in utero electroporation. This method is useful to gain understanding of in vivo function of genes in normal brain development and the changes in gene expression of neuron cells during tumor progression.
06:00
Cortex-, Hippocampus-, Thalamus-, Hypothalamus-, Lateral Septal Nucleus- and Striatum-specific In Utero Electroporation in the C57BL/6 Mouse
0 Views •
Cited by 14 •
2016
This protocol describes in detail how to specifically transfect different regions in the C57BL/6 central nervous system via in utero electroporation. Included in this protocol are detailed instructions for transfections of regions that develop into the cortex, hippocampus, thalamus, hypothalamus, lateral septal nucleus and striatum.
10:08
Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
0 Views •
Cited by 1 •
2017
Many developmentally important genes have cell- or tissue-specific expression patterns. This paper describes LM RNA-seq experiments to identify genes that are differentially expressed at the maize leaf blade-sheath boundary and in lg1-R mutants compared to wild-type. The experimental considerations discussed here apply to transcriptomic analyses of other developmental phenomena.
05:51
Microinjection of Live Drosophila Embryos: Early Delivery of Reagents to the Developing Embryo
0 Views •
2023
This video describes how to perform microinjections that deliver reagents into live Drosophila embryos. Researchers can use this method to introduce exogenous compounds like nucleic acids and proteins during the early stages of embryo development. The featured protocol demonstrates how to set up and perform the procedure for injection of any soluble reagent–here, a fluorescent protein for live imaging experiments.
FAQs
During primordium unrolling, differential growth changes tissue curvature because neighboring regions do not expand identically. Cell proliferation, cell enlargement, and changes in cell shape can make some regions extend faster than others, progressively reducing the original folding or rolling. Measuring these regional differences connects cellular behavior with the developing organ’s changing geometry.
Polarity and mechanical forces help explain why opening is coordinated rather than random. Polarity relates growth and tissue behavior to direction within the developing organ, while mechanical forces influence how expanding regions alter curvature and affect neighboring tissue. Considering both factors allows biologists to interpret the transition as an integrated patterning and tissue-shaping process.
Following the transition provides a link between early tissue patterning and mature organ architecture. The changing form shows how initial spatial organization can be expressed through later growth, rather than treating the mature organ as a structure that appears independently. This perspective is useful when researchers ask how developmental information becomes visible in final organ shape.
Researchers can study Primordium Unrolling by combining developmental imaging with measurements of growth across the tissue. Imaging records how the form changes over time, while regional growth measurements identify where expansion differs. Relating those observations to curvature and cell behavior can reveal how coordinated growth contributes to opening and organ formation.
Useful measurements include regional growth rates, changes in tissue curvature, and cellular behaviors such as proliferation, enlargement, and shape change. Taken together, these observations distinguish a purely descriptive account of opening from an explanation based on how different parts of the primordium grow. They also help identify relationships between local tissue activity and the organ’s overall form.
In plant developmental biology, this process offers a way to examine how early organ precursors acquire the architecture of mature organs. Researchers can use it to connect polarity, local growth patterns, and mechanical influences with visible changes in form. The topic therefore supports investigations of organ formation that span cellular behavior, tissue geometry, and developmental patterning.